State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
College of Forestry, Guangxi University, Nanning 530004, China.
Acta Biochim Biophys Sin (Shanghai). 2023 Jan 25;55(2):237-249. doi: 10.3724/abbs.2022199.
Bamboo is a nontimber woody plant featuring a long vegetative stage and uncertain flowering time. Therefore, the genes belonging to flowering repressors might be essential in regulating the transition from the vegetative to reproductive stage in bamboo. The ( ) gene plays a pivotal role in floral transition and development. However, little is known about the bamboo homologues. In this study, is isolated by analysis of the . transcriptome database. Phylogenetic analysis shows that is closely related to (rice homolog). is ubiquitously expressed in various tissues, predominantly in vegetative tissues. To investigate the function of , is overexpressed in and rice under the influence of the 35S promoter. Overexpression of in causes early flowering and produces abnormal petals and sepals. Quantitative real-time PCR reveals that overexpression in produces an early flowering phenotype by downregulating and upregulating and produces abnormal floral organs by upregulating , and expressions. Simultaneously, overexpression of in rice alters the expressions of flowering-related genes such as , , and and promotes flowering under field conditions. In addition, PvSVP1 may be a nuclear protein which interacts with PvVRN1 and PvMADS56 on the yeast two-hybrid and BiFC systems. Our study suggests that may play a vital role in flowering time and development by interacting with PvVRN1 and PvMADS56 in the nucleus. Furthermore, this study paves the way toward understanding the complex flowering process of bamboo.
竹子是一种非木材木质植物，具有漫长的营养生长阶段和不确定的开花时间。因此，属于开花抑制剂的基因可能在调节竹子从营养生长阶段向生殖阶段的转变中起着至关重要的作用。 （）基因在花的转变和发育中起着关键作用。然而，关于竹子同源物的了解甚少。在这项研究中，通过分析 转录组数据库，分离出了 。系统发育分析表明， 与 （水稻同源物）密切相关。 在各种组织中广泛表达，主要在营养组织中表达。为了研究 的功能，在 的影响下， 在 和水稻中过表达。 在 中的过表达导致早花，并产生异常花瓣和萼片。实时定量 PCR 显示， 在 中的过表达通过下调 和上调 和上调 、 和 的表达来产生早花表型，并产生异常花器官。同时， 在水稻中的过表达改变了与开花相关的基因如 、 、 和 的表达，并在田间条件下促进开花。此外，PvSVP1 可能是一种核蛋白，它在酵母双杂交和 BiFC 系统中与 PvVRN1 和 PvMADS56 相互作用。我们的研究表明， 通过与 PvVRN1 和 PvMADS56 在核内相互作用，可能在开花时间和发育中起重要作用。此外，这项研究为理解竹子复杂的开花过程铺平了道路。
