Isolation and Functional Characterization of Two Homologous Genes from Mango.

The gene is a transcription factor that integrates flowering signals and plays an important role in the regulation of flowering time in many plants. In this study, two full-length cDNA sequences of homologous genes- and -were obtained from 'SiJiMi' mango. Sequence analysis showed that the had typical MADS-box domains and were highly conserved between each other. The analysis of expression patterns showed that the were expressed during flower development and highly expressed in vegetative tissues, with low expression in flowers/buds. The could responded to low temperature, NaCl, and PEG treatment. Subcellular localization revealed that MiSVP1 and MiSVP2 were localized in the nucleus. Transformation of revealed that overexpression of delayed flowering time, overexpression of accelerated flowering time, and neither nor had an effect on the number of rosette leaves. Overexpression of increased the expression of and decreased the expression of and , and overexpression of increased the expression levels of and and decreased the expression levels of . Point-to-point and bimolecular fluorescence complementation (BiFC) assays showed that MiSVP1 and MiSVP2 could interact with SEP1-1, SOC1D, and AP1-2. These results suggest that and may play a significant roles in the flowering process of mango.