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从芒果中分离和功能表征两个同源基因。

Isolation and Functional Characterization of Two Homologous Genes from Mango.

机构信息

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, 100 East Daxue Road, Nanning 530004, China.

出版信息

Int J Mol Sci. 2021 Sep 10;22(18):9802. doi: 10.3390/ijms22189802.

DOI:10.3390/ijms22189802
PMID:34575962
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8471839/
Abstract

The gene is a transcription factor that integrates flowering signals and plays an important role in the regulation of flowering time in many plants. In this study, two full-length cDNA sequences of homologous genes- and -were obtained from 'SiJiMi' mango. Sequence analysis showed that the had typical MADS-box domains and were highly conserved between each other. The analysis of expression patterns showed that the were expressed during flower development and highly expressed in vegetative tissues, with low expression in flowers/buds. The could responded to low temperature, NaCl, and PEG treatment. Subcellular localization revealed that MiSVP1 and MiSVP2 were localized in the nucleus. Transformation of revealed that overexpression of delayed flowering time, overexpression of accelerated flowering time, and neither nor had an effect on the number of rosette leaves. Overexpression of increased the expression of and decreased the expression of and , and overexpression of increased the expression levels of and and decreased the expression levels of . Point-to-point and bimolecular fluorescence complementation (BiFC) assays showed that MiSVP1 and MiSVP2 could interact with SEP1-1, SOC1D, and AP1-2. These results suggest that and may play a significant roles in the flowering process of mango.

摘要

该基因是一种转录因子，整合开花信号，在许多植物的开花时间调控中发挥重要作用。本研究从‘四季蜜芒’中获得了两个同源基因和的全长 cDNA 序列。序列分析表明，具有典型的 MADS 盒结构域，彼此之间高度保守。表达模式分析表明，在花发育过程中表达，在营养组织中高表达，在花/芽中低表达。能够响应低温、NaCl 和 PEG 处理。亚细胞定位表明 MiSVP1 和 MiSVP2 定位于细胞核。转化表明，过表达延迟开花时间，过表达加速开花时间，和既不影响莲座叶的数量。过表达增加的表达，降低的表达和，过表达增加的表达水平和，并降低的表达水平。点对点和双分子荧光互补（BiFC）试验表明，MiSVP1 和 MiSVP2 可以与 SEP1-1、SOC1D 和 AP1-2 相互作用。这些结果表明和可能在芒果的开花过程中发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/47c0d6a4ce00/ijms-22-09802-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/457fd3f57223/ijms-22-09802-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/d82834950b06/ijms-22-09802-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/cbaa21100ecf/ijms-22-09802-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/ac5d6a1d7abc/ijms-22-09802-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/fd9d8a71ad83/ijms-22-09802-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/a9d47527cfc2/ijms-22-09802-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/47c0d6a4ce00/ijms-22-09802-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/457fd3f57223/ijms-22-09802-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/d82834950b06/ijms-22-09802-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/cbaa21100ecf/ijms-22-09802-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/ac5d6a1d7abc/ijms-22-09802-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/fd9d8a71ad83/ijms-22-09802-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/a9d47527cfc2/ijms-22-09802-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/802d/8471839/47c0d6a4ce00/ijms-22-09802-g007.jpg

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