Siguel E N, Chee K M, Gong J X, Schaefer E J
Lipid Metabolism Laboratory, USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111.
Clin Chem. 1987 Oct;33(10):1869-73.
To develop criteria for deficiency of essential fatty acids (EFA), we used capillary-column gas-liquid chromatography to determine fatty acids (percentage of total fatty acids) in plasma obtained in the fasting state from 56 reference subjects and from 10 patients with intestinal fat malabsorption and suspected EFA deficiency. Fatty acid evaluations (percentage of total fatty acids) that allowed for a clear distinction (P less than 0.01) between reference subjects and patients, based on values two standard deviations below or above the reference mean, included values for linoleic acid (18:2w6) below 27%, and values for palmitic acid (16:0), palmitoleic acid (16:1w7), oleic acid (18:1w9), vaccenic acid (18:1w7), and Mead acid (20:3w9) exceeding 21%, 2.6%, 23.3%, 2.1%, and 0.21%, respectively. Ratios of total EFA to total non-EFA of less than 0.60 and of Mead acid to arachidonic acid of greater than 0.025 also served to identify patients, and were not found in reference subjects. Significant inverse correlations between percentages of plasma EFA and plasma mono-unsaturated fatty acids were noted. Our reference-interval data can be used to assess normality of plasma EFA status.
为制定必需脂肪酸（EFA）缺乏的标准，我们使用毛细管柱气液色谱法测定了56名参考对象以及10名患有肠道脂肪吸收不良且疑似EFA缺乏的患者在空腹状态下采集的血浆中的脂肪酸（占总脂肪酸的百分比）。基于低于或高于参考均值两个标准差的值，能够在参考对象和患者之间进行明确区分（P小于0.01）的脂肪酸评估（占总脂肪酸的百分比）包括：亚油酸（18:2w6）低于27%，以及棕榈酸（16:0）、棕榈油酸（16:1w7）、油酸（18:1w9）、反式油酸（18:1w7）和Mead酸（20:3w9）分别超过21%、2.6%、23.3%、2.1%和0.21%。总EFA与总非EFA的比率低于0.60以及Mead酸与花生四烯酸的比率大于0.025也有助于识别患者，而在参考对象中未发现这些情况。我们注意到血浆EFA百分比与血浆单不饱和脂肪酸百分比之间存在显著的负相关。我们的参考区间数据可用于评估血浆EFA状态的正常性。
