Neu N, Craig S W, Rose N R, Alvarez F, Beisel K W
Department of Immunology and Infectious Diseases, Johns Hopkins Medical Institutions, Baltimore, Maryland.
Clin Exp Immunol. 1987 Sep;69(3):566-74.
After infection with Coxsackievirus B3 (CB3), H-2 congenic strains of mice on an A-background develop an immunologically mediated myocarditis associated with autoantibodies directed mainly against cardiac myosin. We tested these autoantibodies for cross-reactivity with the virus. Using immunoblotting and virus neutralizing assays with affinity purified antibodies and absorbed sera, we found that the autoantibodies to heart myosin did not cross react with CB3. In addition, myosin autoantibodies induced by immunization with cardiac myosin did not react with the virus. Western immunoblotting revealed that the anti-CB3 antibodies in the infected mice are directed against the capsid protein VP 2. We conclude that CB3 antigens do not stimulate an immune response that cross-reacts with cardiac myosin.
感染柯萨奇病毒B3（CB3）后，A背景的H-2同源近交系小鼠会发生免疫介导的心肌炎，伴有主要针对心肌肌球蛋白的自身抗体。我们检测了这些自身抗体与该病毒的交叉反应性。通过使用亲和纯化抗体和吸收血清进行免疫印迹及病毒中和试验，我们发现针对心脏肌球蛋白的自身抗体不与CB3发生交叉反应。此外，用心脏肌球蛋白免疫诱导产生的肌球蛋白自身抗体也不与该病毒发生反应。蛋白质免疫印迹显示，感染小鼠体内的抗CB3抗体针对衣壳蛋白VP 2。我们得出结论，CB3抗原不会刺激产生与心脏肌球蛋白发生交叉反应的免疫应答。
