Ahmed Borhan, Hasan Fakhrul, Tabassum Anika, Ahmed Rasel, Hassan Rajnee, Amin Md Ruhul, Alam Mobashwer
Basic and Applied Research On Jute Project, Bangladesh Jute Research Institute, Dhaka, 1207, Bangladesh.
Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur, 1706, Bangladesh.
J Genet Eng Biotechnol. 2023 Jan 18;21(1):5. doi: 10.1186/s43141-022-00453-x.
Sucrose non-fermenting-1 (SNF1)-related protein kinase 2 (SnRK2), a plant-specific serine/threonine kinase family, is associated with metabolic responses, including abscisic acid signaling under biotic and abiotic stresses. So far, no information on a genome-wide investigation and stress-mediated expression profiling of jute SnRK2 is available. Recent whole-genome sequencing of two Corchorus species prompted to identify and characterize this SnRK2 gene family.
We identified seven SnRK2 genes of each of Corchorus olitorius (Co) and C. capsularis (Cc) genomes, with similar physico-molecular properties and sub-group patterns of other models and related crops. In both species, the SnRK2 gene family showed an evolutionarily distinct trend. Highly variable C-terminal and conserved N-terminal regions were observed. Co- and CcSnRK2.3, Co- and CcSnRk2.5, Co- and CcSnRk2.7, and Co- and CcSnRK2.8 were upregulated in response to drought and salinity stresses. In waterlogging conditions, Co- and CcSnRk2.6 and Co- and CcSnRK2.8 showed higher activity when exposed to hypoxic conditions. Expression analysis in different plant parts showed that SnRK2.5 in both Corchorus species is highly expressed in fiber cells providing evidence of the role of fiber formation.
This is the first comprehensive study of SnRK2 genes in both Corchorus species. All seven genes identified in this study showed an almost similar pattern of gene structures and molecular properties. Gene expression patterns of these genes varied depending on the plant parts and in response to abiotic stresses.
蔗糖非发酵-1（SNF1）相关蛋白激酶2（SnRK2）是植物特有的丝氨酸/苏氨酸激酶家族，与代谢反应相关，包括在生物和非生物胁迫下的脱落酸信号传导。到目前为止，关于黄麻SnRK2的全基因组研究和胁迫介导的表达谱分析尚无相关信息。最近两种黄麻属物种的全基因组测序促使人们对该SnRK2基因家族进行鉴定和表征。
我们在长蒴黄麻（Co）和圆果黄麻（Cc）的基因组中各鉴定出7个SnRK2基因，其物理分子特性和亚组模式与其他模式植物和相关作物相似。在这两个物种中，SnRK2基因家族呈现出进化上独特的趋势。观察到高度可变的C末端和保守的N末端区域。CoSnRK2.3和CcSnRK2.3、CoSnRK2.5和CcSnRK2.5、CoSnRK2.7和CcSnRK2.7以及CoSnRK2.8和CcSnRK2.8在干旱和盐胁迫下上调表达。在渍水条件下，CoSnRK2.6和CcSnRK2.6以及CoSnRK2.8和CcSnRK2.8在缺氧条件下表现出更高的活性。在不同植物部位的表达分析表明，两种黄麻属植物中的SnRK2.5在纤维细胞中高度表达，这为纤维形成的作用提供了证据。
这是对两种黄麻属植物中SnRK2基因的首次全面研究。本研究中鉴定出的所有7个基因显示出几乎相似的基因结构和分子特性模式。这些基因的表达模式因植物部位和对非生物胁迫的响应而异。
