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大豆中GmSnRK2家族的全基因组鉴定与特征分析

Genome-Wide Identification and Characterization of the GmSnRK2 Family in Soybean.

作者信息

Zhao Wei, Cheng Yi-Hui, Zhang Chi, Shen Xin-Jie, You Qing-Bo, Guo Wei, Li Xiang, Song Xue-Jiao, Zhou Xin-An, Jiao Yong-Qing

机构信息

Key laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Science, Wuhan 430062, China.

Beijing Genomics Institute, Wuhan 430075, China.

出版信息

Int J Mol Sci. 2017 Aug 23;18(9):1834. doi: 10.3390/ijms18091834.

Abstract

Sucrose non-fermenting-1 (SNF1)-related protein kinase 2s (SnRK2s) that were reported to be involved in the transduction of abscisic acid (ABA) signaling, play important roles in response to biotic and abiotic stresses in plants. Compared to the systemic investigation of in and , little is known regarding in soybean, which is one of the most important oil and protein crops. In the present study, we performed genome-wide identification and characterization of in soybean. In summary, 22 were identified and clustered into four groups. Phylogenetic analysis indicated the expansion of gene family during the evolution of soybean. Various -acting elements such as ABA Response Elements (ABREs) were identified and analyzed in the promoter regions of . The results of RNA sequencing (RNA-Seq) data for different soybean tissues showed that exhibited spatio-temporally specific expression patterns during soybean growth and development. Certain could respond to the treatments including salinity, ABA and strigolactones. Our results provide a foundation for the further elucidation of the function of genes in soybean.

摘要

蔗糖非发酵-1(SNF1)相关蛋白激酶2(SnRK2)据报道参与脱落酸(ABA)信号转导,在植物应对生物和非生物胁迫中发挥重要作用。与对拟南芥和水稻的系统研究相比,对于大豆(最重要的油料和蛋白质作物之一)中SnRK2的了解甚少。在本研究中,我们对大豆中的SnRK2进行了全基因组鉴定和特征分析。总之,鉴定出22个SnRK2并将其分为四组。系统发育分析表明SnRK2基因家族在大豆进化过程中发生了扩张。在SnRK2的启动子区域鉴定并分析了各种顺式作用元件,如ABA反应元件(ABRE)。不同大豆组织的RNA测序(RNA-Seq)数据结果表明,SnRK2在大豆生长发育过程中表现出时空特异性表达模式。某些SnRK2能够响应包括盐度、ABA和独脚金内酯在内的处理。我们的结果为进一步阐明大豆中SnRK2基因的功能奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d2a/5618483/7b2aa7818ff8/ijms-18-01834-g001.jpg

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