Institute of Gene Biology of the Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2023 Feb;508(1):12-16. doi: 10.1134/S160767292270003X. Epub 2023 Jan 18.
To carry out antitumor activity against cells that have lost surface antigens, human lymphocytes must have a certain repertoire of surface proteins capable of contacting a tumor cell and inducing programmed cell death in it. In this work, we showed that activation of healthy donor cells by IL-2 cytokine within 6 days causes the appearance of FasL, CD25, and LFA-1 proteins on CD8+CD25+ T lymphocytes, and also converts the LFA-1 protein into an active form having a high affinity for its target, ICAM-1 integrin. The appearance of these proteins on the surface of this subpopulation of lymphocytes allows them to induce programmed cell death in HLA-negative tumor cells.
为了对失去表面抗原的细胞发挥抗肿瘤活性，人类淋巴细胞必须具有一定的表面蛋白谱，这些蛋白能够与肿瘤细胞接触，并诱导其程序性细胞死亡。在这项工作中，我们表明，在 6 天内，IL-2 细胞因子激活健康供体细胞，会导致 CD8+CD25+T 淋巴细胞表面 FasL、CD25 和 LFA-1 蛋白的出现，并且还会将 LFA-1 蛋白转化为具有高亲和力与其靶标 ICAM-1 整合素结合的活性形式。这些蛋白出现在淋巴细胞的这个亚群表面，使它们能够诱导 HLA 阴性肿瘤细胞的程序性细胞死亡。
