Laboratory of Molecular Immunogenetics of Cancer, Institute of Gene Biology RAS, Vavilova 34/5, 111394 Moscow, Russia.
Cells. 2020 Dec 4;9(12):2602. doi: 10.3390/cells9122602.
Studies on the mechanisms of activation of cytotoxic lymphocyte subpopulations are an important research direction in modern immunology. This study provides a detailed analysis of the effect of Tag7 (PGRP-S, PGLYRP1) on the development of lymphocyte subpopulations cytotoxic against MHC-negative tumor cells in a pool of peripheral blood mononuclear cells (PBMCs). The results show that Tag7 can bind to the TREM-1 receptor on the surfaces of monocytes, thereby triggering the expression of mRNA TNFα and IFNγ. The appearance of these cytokines in conditioned medium leads to IL-2 cytokine secretion by CD3CD4 lymphocytes. In turn, IL-2 facilitates unspecific activation of three cytotoxic cell subpopulations in the PBMC pool: NK (CD16CD56), CD3CD4 and CD3CD8. These subpopulations appear after a certain period of incubation with Tag7 and show toxicity against tumor cells.
研究细胞毒性淋巴细胞亚群的激活机制是现代免疫学的一个重要研究方向。本研究详细分析了 Tag7（PGRP-S，PGLYRP1）对混合外周血单个核细胞（PBMC）中针对 MHC 阴性肿瘤细胞的细胞毒性淋巴细胞亚群发育的影响。结果表明，Tag7 可以与单核细胞表面的 TREM-1 受体结合，从而触发 TNFα 和 IFNγ mRNA 的表达。这些细胞因子在条件培养基中的出现导致 CD3CD4 淋巴细胞分泌 IL-2 细胞因子。反过来，IL-2 促进 PBMC 池中的三种细胞毒性细胞亚群的非特异性激活：NK（CD16CD56）、CD3CD4 和 CD3CD8。这些亚群在与 Tag7 孵育一定时间后出现，并对肿瘤细胞表现出毒性。
