Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs Bd. MDC7, Tampa, FL, 33612, USA.
Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, TX, 77030, USA.
Mol Biotechnol. 2023 Sep;65(9):1476-1484. doi: 10.1007/s12033-023-00663-9. Epub 2023 Jan 18.
Bacteremia poses great risk for morbidity and mortality for immunocompromised cancer patients. Although the presence of bacteria within solid tumors is gaining greater attention, few studies have analyzed species of bacteria in the blood and their effect on cancer clinical outcomes. Using the Kraken 2 taxonomic profiling tool, we classified bacteria present in blood and primary tumors of cervical cancer and melanoma cases. The Cancer Genome Atlas (TCGA) melanoma blood exome files with Pseudomonas species were found to represent a worse disease-free survival (DFS) probability, while a worse overall survival (OS) result was evidenced for both the TCGA and Moffitt Cancer Center melanoma datasets. Cervical cancer cases with reads representing the Bradyrhizobium genus and Bradyrhizobium sp. BTAi1 found in blood and tumor exome files were found to have lower DFS. Additionally, reduced DFS and OS were observed for cervical cancer cases positive for Bacteroides species including Bacteroides fragilis. This study provides novel evidence and a novel approach for indicating that bacteria in blood is associated with cancer recurrence. These findings may guide the development of more efficient prognostic and screening tools related to bacterial blood infections of melanoma and cervical cancer patients.
菌血症会给免疫功能低下的癌症患者带来极大的发病和死亡风险。尽管实体肿瘤内的细菌越来越受到关注，但很少有研究分析血液中的细菌种类及其对癌症临床结果的影响。本研究使用 Kraken 2 分类分析工具，对宫颈癌和黑色素瘤病例的血液和原发肿瘤中的细菌进行分类。在 TCGA 黑色素瘤血液外显子组文件中发现假单胞菌物种代表更差的无病生存（DFS）概率，而 TCGA 和莫菲特癌症中心黑色素瘤数据集均表明更差的总生存（OS）结果。在血液和肿瘤外显子组文件中发现血液和肿瘤外显子组文件中存在根瘤菌属和 Bradyrhizobium sp. BTAi1 的读长的宫颈癌病例具有更低的 DFS。此外，血液中存在拟杆菌属（包括脆弱拟杆菌）的宫颈癌病例的 DFS 和 OS 降低。本研究为血液中的细菌与癌症复发相关提供了新的证据和新的方法。这些发现可能为开发与黑色素瘤和宫颈癌患者的细菌血液感染相关的更有效的预后和筛查工具提供指导。
