Department of Microbiology, University of Central Punjab Lahore, Pakistan.
Department of Microbiology, Pakistan Kidney and Liver Institute and Research Center, Lahore, Pakistan.
Asian Pac J Cancer Prev. 2020 May 1;21(5):1333-1338. doi: 10.31557/APJCP.2020.21.5.1333.
The objectives of this study were to evaluate P. Aeruginosa isolates from cancer patients for the phenotypic pattern of antibiotic resistance and to detect the gene responsible for virulence as well as antibiotic resistance.
A total of 227 P. aeruginosa isolates were studied and 11 antibiotics were applied for susceptibility testing. PCR detection of the genes BIC, TEM, IMP, SPM, AIM, KPC, NDM, GIM, VIM, OXA, toxA and oprI was done. Finally, the carbapenem resistant isolates were tested for phenotypic identification of carbapenemase enzyme by Modified Hodge test.
The results showed that the isolates were resistant to imipenem (95%), cefipime (93%), meropenem (90%), polymixin B (71%), gentamicin (65%), ciprofloxacin (48%), ceftazidime (40%), levofloxacin (39%), amikacin (32%), tobramycin (28%) and tazobactum (24%). The PCR detection of the carbapenem resistant genes showed 51% isolates were positive for IMP, GIM and VIM, 38% for AIM and SPM, 30% for BIC, 20% for TEM and NDM, 17% for KPC and 15% for OXA. However, toxA and oprI genes were not detected. 154 carbapenem resistant isolates were found positive phenotypically for carbapenemase enzyme identification by Modified Hodge test.
The co-existence of multiple drug-resistant bodies and virulent genes has important implications for the treatment of patients. This study provides information about treating drug-resistant P. Aeruginosa and the relationship of virulent genes with phenotypic resistance patterns.
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本研究旨在评估癌症患者的铜绿假单胞菌分离株的表型抗生素耐药模式，并检测与毒力以及抗生素耐药相关的基因。
研究共纳入 227 株铜绿假单胞菌，应用 11 种抗生素进行药敏试验。应用 PCR 检测 BIC、TEM、IMP、SPM、AIM、KPC、NDM、GIM、VIM、OXA、toxA 和 oprI 基因。最后，采用改良 Hodge 试验对碳青霉烯类耐药株进行碳青霉烯酶表型鉴定。
结果显示，分离株对亚胺培南（95%）、头孢吡肟（93%）、美罗培南（90%）、多黏菌素 B（71%）、庆大霉素（65%）、环丙沙星（48%）、头孢他啶（40%）、左氧氟沙星（39%）、阿米卡星（32%）、妥布霉素（28%）和他唑巴坦（24%）耐药。PCR 检测碳青霉烯耐药基因显示，51%的分离株 IMP、GIM 和 VIM 基因阳性，38%的分离株 AIM 和 SPM 基因阳性，30%的分离株 BIC 基因阳性，20%的分离株 TEM 和 NDM 基因阳性，20%的分离株 KPC 基因阳性，17%的分离株 OXA 基因阳性。然而，未检测到 toxA 和 oprI 基因。154 株碳青霉烯类耐药分离株经改良 Hodge 试验阳性，鉴定为碳青霉烯酶。
多种耐药体和毒力基因的共存对患者的治疗具有重要意义。本研究为治疗耐药铜绿假单胞菌和毒力基因与表型耐药模式的关系提供了信息。
