Harvey Faith Christine, Collao Vanessa, Bhattacharya Sanjoy K
Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Miami Integrative Metabolomics Research Center, Miami, FL, USA.
Methods Mol Biol. 2023;2625:57-63. doi: 10.1007/978-1-0716-2966-6_4.
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is a powerful tool for identification and classification of lipids. Ultra-high performance liquid chromatography (UHPLC) allows for robust separations of complex mixtures, while high-resolution mass spectrometry (HRMS) identifies compounds with efficiency and accuracy (Zullig T and Kofeler HC, Mass Spectrom Rev 40:162-176, 2021). The high specificity and sensitivity of mass spectrometry makes it the method of choice when analyzing lipids (Kofeler HC, J Lipid Res 62:100138, 2021). Untargeted mass spectrometry identifies all lipids within a sample and is useful for identification and further discovery. This chapter describes the use of a Q Exactive mass spectrometer to perform an untargeted LC-MS/MS lipidomics analysis.
液相色谱-串联质谱联用（LC-MS/MS）是用于脂质鉴定和分类的强大工具。超高效液相色谱（UHPLC）能够对复杂混合物进行稳健分离，而高分辨率质谱（HRMS）可高效且准确地鉴定化合物（祖利格T和科费勒HC，《质谱评论》40:162 - 176，2021年）。质谱的高特异性和灵敏度使其成为分析脂质时的首选方法（科费勒HC，《脂质研究杂志》62:100138，2021年）。非靶向质谱可鉴定样品中的所有脂质，有助于鉴定和进一步发现。本章介绍使用Q Exactive质谱仪进行非靶向LC-MS/MS脂质组学分析的方法。
