Ren Fan, Miao Ran, Xiao Rui, Mei Jie
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Frontier Science Center for Immunology and Metabolism, Medical Research Institute, Wuhan University, Wuhan 430071, China; Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China.
Sci Bull (Beijing). 2021 Jun 15;66(11):1119-1128. doi: 10.1016/j.scib.2021.02.003. Epub 2021 Feb 6.
Bucky ball (Buc) is involved in germ plasm (GP) assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism. The present study demonstrates that an mA reader Igf2bp3 interacts and colocalizes with Buc in the GP. Similar to the loss of Buc, the genetic deletion of maternal igf2bp3 in zebrafish leads to abnormal GP assembly and insufficient germ cell specification, which can be partially restored by the injection of igf2bp3 mRNA. Igf2bp3 binds to mA-modified GP-organizer and GP mRNAs in an mA-dependent manner and prevents their degradation. These findings indicate that the functions of Igf2bp3, a direct effector protein of Buc, in GP mRNA expression and GP assembly involve mA-dependent regulation; these results emphasize a critical role of mA modification in the process of GP assembly.
巴基球（Buc）在斑马鱼早期发育过程中通过一种未知机制调节生殖质（GP）mRNA的表达，从而参与GP的组装。本研究表明，一种mA阅读器Igf2bp3在GP中与Buc相互作用并共定位。与Buc缺失类似，斑马鱼中母源igf2bp3的基因缺失导致GP组装异常和生殖细胞特化不足，而注射igf2bp3 mRNA可部分恢复这种情况。Igf2bp3以mA依赖的方式与mA修饰的GP组织者和GP mRNA结合，并防止它们降解。这些发现表明，作为Buc直接效应蛋白的Igf2bp3在GP mRNA表达和GP组装中的功能涉及mA依赖的调节；这些结果强调了mA修饰在GP组装过程中的关键作用。
