Yadav Kusum, Singh Tejasvita, Varma Kachnar, Bhargava Mudita, Misra Vatsala
Department of Pathology, M.L.N. Medical College, Prayagraj, Uttar Pradesh, India.
Indian J Pathol Microbiol. 2023 Jan-Mar;66(1):3-8. doi: 10.4103/ijpm.ijpm_190_22.
To evaluate the association of tumor budding (TB) with prognostic histomorphological parameters in oral squamous cell carcinoma (OSCC) and to investigate the correlation of TB intensity with epithelial to mesenchymal transition (EMT).
A total of 200 cases diagnosed as OSCC were selected and their TB status was reviewed using Hematoxylin and eosin (H and E) and Immunohistochemistry (IHC). Correlation with histomorphological prognostic parameters was done. Also, IHC for Vimentin and E-cadherin was performed to look for EMT.
On H and E examination, TB was observed in 154/200 (77%). About 88/154 (57.14%) cases showed a high TB (>5 TB/10 hpf) which increased to 100/154 (64.9%) cases on IHC staining. The intensity of TB was significantly associated with tumor grade and depth of invasion. It was also significantly associated with reduced expression for E-Cadherin and upregulation of Vimentin establishing a pathogenetic correlation between the TB and EMT.
Therefore, our results suggest that TB is associated with poor prognosis and histologically represents EMT in OSCC which further adds to the aggressiveness of the tumor.
评估口腔鳞状细胞癌（OSCC）中肿瘤芽生（TB）与预后组织形态学参数的相关性，并研究TB强度与上皮-间质转化（EMT）的相关性。
选取200例诊断为OSCC的病例，采用苏木精-伊红（H&E）染色和免疫组织化学（IHC）检查其TB状态。分析其与组织形态学预后参数的相关性。同时，进行波形蛋白和E-钙黏蛋白的免疫组织化学检测以寻找EMT。
在H&E检查中，200例中有154例（77%）观察到TB。约88/154例（57.14%）显示高TB（>5个TB/10高倍视野），免疫组织化学染色后增加至100/154例（64.9%）。TB强度与肿瘤分级和浸润深度显著相关。它还与E-钙黏蛋白表达降低和波形蛋白上调显著相关，从而在TB与EMT之间建立了发病机制上的关联。
因此，我们的结果表明，TB与预后不良相关，并且在组织学上代表OSCC中的EMT，这进一步增加了肿瘤的侵袭性。
