Ji Na, Wang Nan, Liu Gang, Zhao Shanshan, Liu Zhiqiang, Tan Wenbo, Wang Shiyi, Sheng Jinjiang, Li Fengshi, Wang Yuanzhi
Department of Basic Medicine, School of Medicine, Shihezi University, Shihezi City, Xinjiang Uygur Autonomous Region 832002, People's Republic of China.
NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi City, Xinjiang Uygur Autonomous Region 832002, People's Republic of China.
J Wildl Dis. 2023 Jan 1;59(1):138-142. doi: 10.7589/JWD-D-21-00137.
Tacheng tick virus 1 (TcTV-1) and Songling virus (SGLV) were originally found in human patients in China who had had tick bites. Tamdy virus (TAMV) was detected for the first time in a tick-infested person from Kyrgyzstan in 1973. In this study, 276 great gerbils (Rhombomys opimus) were collected in Xinjiang Uygur Autonomous Region in northwestern China. The total RNA of individual spleen samples was extracted, and the viral L segments of TcTV-1, SGLV, and TAMV were detected by nested reverse transcription PCR. Overall, 2.9% (8/276) and 2.2% (6/276) of spleen samples tested positive to the viral L segments for TcTV-1 and SGLV, respectively; TAMV was not detected in any samples. The SGLV from the great gerbils shared 93.7% (236/252 nucleotide [nt]) and 94.0% (78/83 amino acid [aa]) identities to SGLV detected in patients infected with SGLV in northeastern China. The TcTV-1 in great gerbils was closest to TcTV-1 from a patient in China, with 98.5% (797/809 nt) and 98.9% (265/268 aa) sequence identities. This is the first molecular evidence for the presence of TcTV-1 and SGLV in great gerbils. High genetic diversity in SGLV was observed among geographical locations. Multiregion surveillance of Tamdy orthonairoviruses in more wildlife species is necessary.
塔城蜱病毒1型（TcTV-1）和松岭病毒（SGLV）最初在中国被蜱叮咬过的人类患者中发现。1973年，在吉尔吉斯斯坦一名受蜱虫侵扰的人身上首次检测到坦迪病毒（TAMV）。在本研究中，在中国西北部的新疆维吾尔自治区采集了276只大沙鼠（子午沙鼠）。提取了各个脾脏样本的总RNA，并通过巢式逆转录PCR检测了TcTV-1、SGLV和TAMV的病毒L片段。总体而言，分别有2.9%（8/276）和2.2%（6/276）的脾脏样本对TcTV-1和SGLV的病毒L片段检测呈阳性；在任何样本中均未检测到TAMV。大沙鼠身上的SGLV与在中国东北部感染SGLV的患者中检测到的SGLV的核苷酸同源性为93.7%（236/252个核苷酸[nt]），氨基酸同源性为94.0%（78/83个氨基酸[aa]）。大沙鼠身上的TcTV-1与来自中国一名患者的TcTV-1最为接近，序列同源性分别为98.5%（797/809 nt）和98.9%（265/268 aa）。这是大沙鼠中存在TcTV-1和SGLV的首个分子证据。在不同地理位置观察到SGLV存在高度遗传多样性。有必要对更多野生动物物种中的坦迪正布尼亚病毒进行多区域监测。
