The term exercise-induced cardiac fatigue (EICF) has typically been used to describe a transient reduction in cardiac function following prolonged-strenuous exercise. Recent evidence demonstrates that EICF can occur following only 45 min of high-intensity exercise when assessed using exercising stress echocardiography. This investigation sought to examine whether sprint intervals (SIT; 6 × 30 s Wingate tests), or 90-min moderate-cycling with sprint intervals (MIX; 90 min with 1 × 30 s Wingate test every 15 min) would cause greater EICF than 90 min (CON) or 3 h (LONG) moderate-cycling assessed using stress echocardiography, with a secondary aim to interrogate sex differences in EICF. Seventeen participants (M: 9, F: 8) underwent three cycling sessions with stress-echocardiography performed before-and-after each condition at a target heart rate (HR) of 100 beats·min, with the CON testing occurring at the mid-point of the 3 h LONG condition. For all conditions, measures of left ventricular (LV) systolic [stroke volume, ejection fraction (EF), peak longitudinal strain, isovolumetric contraction time, ') and diastolic (/, ', isovolumetric relaxation time, longitudinal strain rate) function were reduced after exercise (all < 0.05). In the right ventricle (RV), systolic function was reduced (tricuspid annular plane systolic excursion, ', peak longitudinal strain and strain rate) following all conditions, and fractional area change was reduced to the greatest degree following SIT (condition × time, = 0.01). Females demonstrated lesser impairments in LV EF, and elastance (ESP/ESV) compared with males ( < 0.05). Markers of EICF occurred similarly following all cycling loads, suggesting the functional changes may be due to altered loading conditions and reduced stress-echocardiography workload. However, males experienced greater cardiac alterations in some measures, likely due to greater changes in postexercise loading conditions. This investigation sought to determine the role of exercise intensity on the magnitude of exercise-induced cardiac fatigue using stress echocardiography to maintain loading conditions, with a secondary purpose of assessing sex differences. Unexpectedly, it was found that all cycling loads elicited the same magnitude of functional alteration, which likely represents a common response to exercise and stress echocardiography, rather than intrinsic cardiac impairment. Males demonstrated greater alterations than females, likely due to sex differences in postexercise hemodynamics.