Department of Biology, College of Sciences and Humanities, Prince Sattam Bin Abdulaziz University, P.O. Box: 83, Al-Kharj 11940, Saudi Arabia.
Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, P.O. Box 6830, Riyadh 11452, Saudi Arabia.
Forensic Sci Int. 2023 Feb;343:111562. doi: 10.1016/j.forsciint.2023.111562. Epub 2023 Jan 7.
This research explores DNA consistency and attempts to detect STR profiles from the degrading menstrual blood samples (MBS) as reliable forensic evidence. Peripheral (PBS) and MBS of 30 healthy fertile females were taken on the menstrual cycle's second day. They were obtained at different time periods (0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 24, and 48 h) at 25 °C. DNA evaluation was fulfilled to analyze DNA profiles. A considerable elevation in the median concentrations of DNA between 0 and 14-h intervals were documented, whereas decreased extents were registered between 16 and 48 h. Moreover, complete STR profiles (24/24) for DNA were discovered in all the intervals (0, 2, and 48 h). Periods of 0-8 h demonstrated the maximum extents of DNA materials. Full STR were discovered in all the intervals (0, 2, and 48 h). Eventually, MBS can be utilized as forensic evidence.
本研究旨在探索 DNA 的一致性，并尝试从降解的月经血样本 (MBS) 中检测 STR 图谱作为可靠的法医证据。采集了 30 名健康生育期女性在月经周期第二天的外周血 (PBS) 和 MBS。将它们在 25°C 下于不同时间段（0、2、4、6、8、10、12、14、16、18、20、24 和 48 小时）获取。进行 DNA 评估以分析 DNA 图谱。结果表明，0 至 14 小时之间的 DNA 中位浓度显著升高，而在 16 至 48 小时之间则有所下降。此外，在所有间隔时间（0、2 和 48 小时）均发现了完整的 STR 图谱（24/24）。0-8 小时期间的 DNA 物质含量达到最大值。在所有间隔时间（0、2 和 48 小时）均发现了完整的 STR。最终，MBS 可以用作法医证据。
