Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, PR China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China.
Carbohydr Res. 2023 Jan;523:108737. doi: 10.1016/j.carres.2022.108737. Epub 2022 Dec 26.
Steviol glycosides have attracted great interest because of their high levels of sweetness and safety, and absence of calories. Improvement of their sensory qualities via glycosylation modification by glycosyltransferase is a research hotspot. In this study, YjiC, a uridine diphosphate-dependent glycosyltransferase from Bacillus subtilis 168, was found with the ability to glycosylate rebaudioside A (Reb A) to produce a novel mono β-1, 6-glycosylated Reb A derivative rebaudioside L2 (Reb L2). It has an improved sweetness compared with Reb A. Next, a cascade reaction was established by combining YjiC with sucrose synthase AtSuSy from Arabidopsis thaliana for scale-up preparation of Reb L2. It shows that Reb L2 (30.94 mg/mL) could be efficiently synthesized with an excellent yield of 91.34% within 12 h. Therefore, this study provides a potential approach for the production and application of new steviol glycoside Reb L2, expanding the scope of steviol glycosides.
甜菊糖苷因其高甜度、安全性和无热量而引起了极大的关注。通过糖基转移酶的糖基化修饰来改善其感官品质是一个研究热点。本研究发现，枯草芽孢杆菌 168 来源的尿苷二磷酸依赖性糖基转移酶 YjiC 具有将甜菊苷 A（Reb A）糖基化生成新型单-β-1,6-糖苷化甜菊苷 A 衍生物瑞鲍迪苷 L2（Reb L2）的能力，其甜度优于 Reb A。随后，通过将 YjiC 与拟南芥蔗糖合酶 AtSuSy 组合建立级联反应，用于 Reb L2 的规模化制备。结果表明，在 12 h 内可高效合成 Reb L2（30.94 mg/mL），产率高达 91.34%。因此，本研究为新型甜菊糖苷 Reb L2 的生产和应用提供了一种潜在的方法，拓展了甜菊糖苷的应用范围。
