Acet Ömür, Ali Noma Samir Abbas, Acet Burcu Önal, Dikici Emrah, Osman Bilgen, Odabaşı Mehmet
Vocational School of Health Science, Pharmacy Services Program, Tarsus University, Tarsus, Turkey.
Faculty of Arts and Science, Department of Chemistry, Bursa Uludağ University, Bursa, Turkey.
J Pharm Biomed Anal. 2023 Mar 20;226:115250. doi: 10.1016/j.jpba.2023.115250. Epub 2023 Jan 14.
In this study, a L-asparagine (L-Asn) imprinted membranes (L-Asn-MIPs) were synthesized via molecular imprinting for selective and efficient removal of L-Asn. The L-Asn-MIP membrane was prepared by using acrylamide (AAm) and hydroxyethyl methacrylate (HEMA) as a functional monomer and a comonomer, respectively. The membrane was characterized by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The L-Asn adsorption capacity of the membrane was investigated in detail. The maximum L-Asn adsorption capacity was determined as 408.2 mg/g at pH: 7.2, 24 °C. Determination of L-Asn binding behaviors of L-Asn-MIPs also shown with Scatchard analyses. The effect of pH on L-Asn adsorption onto the membrane and also the selectivity and reusability of the L-Asn-MIPs for L-Asn adsorption were determined through L-asparaginase (L-ASNase) enzyme activity measurements. The selectivity of the membrane was investigated by using two different ternary mixtures; L-glycine (L-Gly)/L-histidine (L-His)/L-Asn and L-tyrosin (L-Tyr)/L-cystein(L-Cys)/L-Asn. The obtained results showed that the L-Asn-MIP membranes have a high selectivity towards L-Asn.
在本研究中，通过分子印迹合成了L-天冬酰胺（L-Asn）印迹膜（L-Asn-MIPs），用于选择性高效去除L-天冬酰胺。L-Asn-MIP膜分别以丙烯酰胺（AAm）和甲基丙烯酸羟乙酯（HEMA）作为功能单体和共聚单体来制备。通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）对该膜进行了表征。详细研究了该膜对L-天冬酰胺的吸附容量。在pH值为7.2、温度为24℃时，测得最大L-天冬酰胺吸附容量为408.2 mg/g。通过Scatchard分析也显示了L-Asn-MIPs对L-天冬酰胺的结合行为。通过L-天冬酰胺酶（L-ASNase）活性测定，确定了pH对L-天冬酰胺在膜上吸附的影响以及L-Asn-MIPs对L-天冬酰胺吸附的选择性和可重复使用性。使用两种不同的三元混合物研究了该膜的选择性；L-甘氨酸（L-Gly）/L-组氨酸（L-His）/L-天冬酰胺和L-酪氨酸（L-Tyr）/L-半胱氨酸（L-Cys）/L-天冬酰胺。所得结果表明，L-Asn-MIP膜对L-天冬酰胺具有高选择性。
