Department of Post-Graduate Studies & Research in Chemistry, Mangalore University, Mangalagangothri, 574199 D.K., Karnataka, India.
Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575018, Karnataka, India.
Int J Biol Macromol. 2023 Mar 31;232:123278. doi: 10.1016/j.ijbiomac.2023.123278. Epub 2023 Jan 16.
In this study, the Halloysite nanotubes (HNTs) are surface-functionalized with sodium alginate (Sod.alg) and poly (vinyl alcohol) (PVA) were employed to generate nanocomposite films (Sod.alg-rHNT/PVA). These nanocomposite films were made via the solution casting technique. FE-SEM data verified sod.alg-rHNT dispersion into the PVA matrix. The modifications were confirmed from FTIR, TGA and PXRD techniques. In the mechanical studies of synthesized nanocomposite films, the films showed a considerable increase in the tensile strength and Young's modulus values. The nanocomposite film's ability to induce cell proliferation and migration was investigated using murine fibroblast (NIH3T3) cells. The films increased cellular proliferation (128 ± 1.07 %) compared to the neat PVA. Cell adhesion tests showed cytocompliant films. In the scratch assay, the 5 wt% film elicited wound closure at a faster rate (91.53 ± 1.04 %). Films were compatible with human blood cells. Therefore the prepared nanocomposite films can be used as promising wound healers after pre-clinical and clinical testing.
在这项研究中，用海藻酸钠（Sod.alg）和聚乙烯醇（PVA）对埃洛石纳米管（HNTs）进行表面功能化，以制备纳米复合薄膜（Sod.alg-rHNT/PVA）。这些纳米复合薄膜是通过溶液浇铸技术制备的。FE-SEM 数据证实了 Sod.alg-rHNT 在 PVA 基体中的分散。FTIR、TGA 和 PXRD 技术证实了修饰。在合成纳米复合薄膜的力学研究中，薄膜的拉伸强度和杨氏模量值有了显著提高。使用鼠成纤维细胞（NIH3T3）研究了纳米复合薄膜诱导细胞增殖和迁移的能力。与纯 PVA 相比，薄膜能显著提高细胞增殖（128±1.07%）。细胞黏附试验表明薄膜具有细胞顺应性。在划痕实验中，5wt%的薄膜以更快的速度（91.53±1.04%）诱导伤口闭合。薄膜与人血细胞相容。因此，在经过临床前和临床测试后，这些制备的纳米复合薄膜有望成为有前途的伤口愈合剂。
