Department of Post-Graduate Studies & Research in Chemistry, Mangalore University, Mangalagangothri, 574199 D.K., Karnataka, India.
Department of Chemistry, P.A. College of Engineering, Mangalore, 574153 D.K., Karnataka, India.
Int J Biol Macromol. 2020 Dec 15;165(Pt A):1079-1092. doi: 10.1016/j.ijbiomac.2020.09.188. Epub 2020 Sep 28.
The present study reports the preparation of novel surface functionalized halloysite nanotubes (HNTs) with chitosan incorporated Poly (vinyl alcohol) (PVA) nanocomposite films with desirable properties. Surface functionalization of HNTs with Chitosan through hydrogen bonding via acylation with succinic anhydride; supramolecular interaction was confirmed by spectroscopic and morphological analysis. The functionalized HNTs incorporated in the PVA matrix were subjected to FTIR studies, Atomic Force Microscopy, Scanning Electron Microscopy, X-ray diffraction, thermal, mechanical properties, Water Contact Angle, swelling ratio analysis and in-vitro biocompatibility studies. Results of the morphological studies showed that functionalized HNTs were uniformly dispersed and showed improved surface roughness with increasing weight percent of functionalized HNTs in the films. The studies revealed significant enhancement in the mechanical and thermal properties compared with the pristine PVA film. The hydrophilic or hydrophobic nature of films were analysed with WCA and results were compared with swelling studies. Furthermore, in vitro enzymatic degradation and cellular behaviour studies performed on mouse fibroblast (NIH3T3) cells and results confirmed enhanced proliferative and adhesion activity of nanocomposite films compared to that of pristine PVA films. In addition, hemocompatibility studies carried out using human erythrocytes revealed the biocompatible and hemocompatible of nanocomposite films indicating their greater potential for tissue engineering.
本研究报告了通过琥珀酸酐酰化作用通过氢键对壳聚糖进行表面功能化的新型表面功能化埃洛石纳米管（HNTs）与壳聚糖复合聚（乙烯醇）（PVA）纳米复合材料的制备，具有理想的性能。通过光谱和形态分析证实了 HNTs 与壳聚糖的超分子相互作用。将功能化的 HNTs 掺入 PVA 基质中进行 FTIR 研究、原子力显微镜、扫描电子显微镜、X 射线衍射、热学、力学性能、水接触角、溶胀比分析和体外生物相容性研究。形态研究的结果表明，随着薄膜中功能化 HNTs 重量百分比的增加，功能化 HNTs 均匀分散并显示出改善的表面粗糙度。与原始 PVA 薄膜相比，研究表明机械和热性能得到了显著提高。通过 WCA 分析和结果与溶胀研究进行比较来分析薄膜的亲水性或疏水性。此外，在小鼠成纤维细胞（NIH3T3）上进行的体外酶降解和细胞行为研究证实，与原始 PVA 薄膜相比，纳米复合材料薄膜具有增强的增殖和粘附活性。此外，使用人红细胞进行的血液相容性研究表明纳米复合材料薄膜具有生物相容性和血液相容性，表明它们在组织工程中的潜力更大。
