Muggleton-Harris A L, Hardy K, Higbee N
MRC Experimental Embryology and Teratology Unit, Medical Research Council Laboratories, Carshalton, Surrey, UK.
Development. 1987 Apr;99(4):473-80. doi: 10.1242/dev.99.4.473.
In the study of the lens of a congenital cataractous mouse mutant (CAT), it has been shown that a loss of growth regulation at the cellular level causes gross lens abnormalities. The phenotypic characteristics of the cataractous mouse lens are similar to those seen in human congenital cataract and thus serves as a model system for medical research. In this present investigation, we have demonstrated that the abnormalities of the congenital cataractous lens can be rescued by forming chimaeras between DBA/2 (a noncataractous strain of mouse) and the CAT mutant. This report describes the histological, cellular and biochemical analysis of the resultant chimaeric eyes, and discusses possible mechanisms by which these results were achieved.
在对一种先天性白内障小鼠突变体（CAT）晶状体的研究中，已表明细胞水平上生长调节的丧失会导致晶状体出现严重异常。白内障小鼠晶状体的表型特征与人类先天性白内障相似，因此可作为医学研究的模型系统。在本次研究中，我们已证明通过在DBA/2（一种无白内障的小鼠品系）和CAT突变体之间形成嵌合体，可以挽救先天性白内障晶状体的异常。本报告描述了所得嵌合体眼睛的组织学、细胞和生化分析，并讨论了取得这些结果的可能机制。
