Muggleton-Harris A L, Higbee N
MRC Experimental Embryology and Teratology Unit, MRC Laboratories, Carshalton, Surrey, U.K.
Exp Eye Res. 1987 Jun;44(6):805-15. doi: 10.1016/s0014-4835(87)80043-x.
A detailed histological study of the embryonic and adult congenital cataract Lop mouse lens has been made. A comparison with the congenital cataract (CatFr) mouse lens has shown that the full range of lens anomalies noted in the congenital cataractous mice are very similar. However, the Lop mutant demonstrates these defects to a greater degree in both the embryonic and adult stages. The lens epithelial cells of the Lop adult lens have been cultured to ascertain their in vitro phenotype. These studies have shown that the proliferation pattern and population doubling level are similar to that of the CatFr lens epithelial cells. A comparison with non-cataractous mouse lens epithelial cells demonstrates that the congenital cataractous mice lens epithelial cells have a very limited life span in vitro. An increase in the nuclear diameter with population doubling level was observed in the cultured mouse lens epithelial cells.
已对胚胎期和成年期先天性白内障Lop小鼠晶状体进行了详细的组织学研究。与先天性白内障（CatFr）小鼠晶状体的比较表明，先天性白内障小鼠中观察到的晶状体异常的全部范围非常相似。然而，Lop突变体在胚胎期和成年期都更严重地表现出这些缺陷。已对Lop成年晶状体的晶状体上皮细胞进行培养，以确定其体外表型。这些研究表明，增殖模式和群体倍增水平与CatFr晶状体上皮细胞相似。与非白内障小鼠晶状体上皮细胞的比较表明，先天性白内障小鼠晶状体上皮细胞在体外的寿命非常有限。在培养的小鼠晶状体上皮细胞中观察到核直径随群体倍增水平增加。
