Li Hui, Gao Tao, Zhang Zhonghao, Lei Jiangping, Hu Junchao, Tang Zizhong, Feng Shiling, Ding Chunbang, Chen Tao, Chen Yanger, Yuan Shu, Yuan Ming
College of Life Science, Sichuan Agricultural University, Ya'an 625014, Sichuan Province, China.
College of Resources, Sichuan Agricultural University, Chengdu 611134, Sichuan Province, China.
Carbohydr Polym. 2023 Mar 1;303:120481. doi: 10.1016/j.carbpol.2022.120481. Epub 2022 Dec 21.
Polysaccharides were the key ingredients of many herbal medicines, and were responsible for multiple pharmacological activities. In this study, a novel polysaccharide fraction, named SLP-2, was isolated from Stauntonia leucantha fruits, and purified by DEAE-52 and Sephadex G-100 column chromatography. Furthermore, SLP-2 was identified by congo red, methylation, partial acid hydrolysis and NMR. The results indicated that the backbone of SLP-2 was composed of →4)-β-D-Galp-(1 → 4)-β-D-Galp-(1→ substituted at C-6 with 1,5-linked arabinan. SLP-2 had good anti-oxidation ability in vitro. Surprisingly, we found that reduction of carboxyl groups and methylation of hydroxyl groups enhanced the ability to scavenge 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals and inhibit lipid peroxidation, and weakened the activity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and reduce ferric iron.
多糖是许多草药的关键成分，并具有多种药理活性。在本研究中，从野木瓜果实中分离出一种名为SLP-2的新型多糖组分，并通过DEAE-52和Sephadex G-100柱色谱法进行纯化。此外，通过刚果红、甲基化、部分酸水解和核磁共振对SLP-2进行了鉴定。结果表明，SLP-2的主链由→4)-β-D-吡喃半乳糖-(1→4)-β-D-吡喃半乳糖-(1→组成，在C-6位被1,5-连接的阿拉伯聚糖取代。SLP-2在体外具有良好的抗氧化能力。令人惊讶的是，我们发现羧基的还原和羟基的甲基化增强了清除2,2'-偶氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基和抑制脂质过氧化的能力，并削弱了清除1,1-二苯基-2-苦基肼(DPPH)自由基和还原三价铁的活性。
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