从辣木（Moringa oleifera）叶中分离得到的阿拉伯半乳聚糖的结构解析及抗氧化活性研究。
Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.
机构信息
Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
出版信息
Int J Biol Macromol. 2018 Jun;112:126-133. doi: 10.1016/j.ijbiomac.2018.01.110. Epub 2018 Jan 31.
A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography. High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 10 Da. Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12. Structural analysis indicated that MOP-1 has a backbone of →1)-β-d-Galp-(3,4→ with highly branched chains at O-4 position. The branches were composed of →1)-β-d-Galp-(4→, →1)-α-d-Galp-(2→, Araf-(1→, Galp-(1→. Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP). The results indicated MOP-1 exhibited significant antioxidant activities.
一种新型阿拉伯半乳聚糖（MOP-1）从辣木（Moringa oleifera）叶片中分离得到，并通过大孔树脂、DEAE-52 纤维素和 Sepharose 6B 凝胶过滤层析进行纯化。高效凝胶渗透色谱（HPGPC）分析表明，MOP-1 的分子量为 7.65×10 Da。单糖组成分析表明，糖组成的摩尔比为 Rha: Ara: Gal=1:7.32:12.12。结构分析表明，MOP-1 具有→1）-β-d-Galp-（3,4→的主链，在 O-4 位置具有高度支化的支链。支链由→1）-β-d-Galp-（4→、→1）-α-d-Galp-（2→、Araf-（1→、Galp-（1→组成。其抗氧化活性通过 DPPH 自由基清除能力、2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基阳离子清除能力和铁还原抗氧化能力（FRAP）进行评估。结果表明，MOP-1 表现出显著的抗氧化活性。
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