An AIEgens and exonuclease III aided quadratic amplification assay for detecting and cellular imaging of telomerase activity.

Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens modified probe (TPE-Py-DNA) as a fluorescence reporter and exonuclease III (Exo III) as a signal amplifier. With the aid of telomerase, repeat units (TTAGGG)n are extended from the end of template substrate oligonucleotides (TS primer) that form duplex DNAs with TPE-Py-DNA. Then, Exo III catalyzes the digestion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1:N. This strategy exhibits good performance for detecting clinical urine samples (distinguishing 15 cancer patients' samples from 8 healthy ones) and checking intracellular telomerase activity (differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug), which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.