Lomonosov Moscow State University, Department of Chemistry, Moscow, 119991, Russia.
Biochemistry (Mosc). 2020 Feb;85(2):147-166. doi: 10.1134/S0006297920020030.
Recently, there has been a rapid progress in the development of techniques for isothermal amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The advantage of these methods is that the nucleic acids amplification can be carried out at constant temperature, unlike PCR, which requires cyclic temperature changes. Moreover, isothermal amplification can be conducted directly in living cells. This review describes the principles of isothermal amplification techniques and demonstrates their high efficiency in designing new highly sensitive detection methods of nucleic acids and enzymes involved in their modifications. The data on successful application of isothermal amplification methods for the analysis of cells and biomolecules with the use of DNA/RNA aptamers are presented.
最近，作为聚合酶链反应（PCR）的替代方法，核酸等温扩增技术取得了快速发展。这些方法的优点是核酸扩增可以在恒温下进行，不像 PCR 需要循环温度变化。此外，等温扩增可以直接在活细胞中进行。本文综述了等温扩增技术的原理，并展示了它们在设计新型高灵敏度核酸检测方法和参与其修饰的酶方面的高效性。介绍了使用 DNA/RNA 适体成功应用等温扩增方法分析细胞和生物分子的相关数据。
