Church W H, Justice J B, Byrd L D
Department of Chemistry, Emory University, Atlanta, GA 30322.
Eur J Pharmacol. 1987 Jul 23;139(3):345-8. doi: 10.1016/0014-2999(87)90592-9.
A microdialysis/smallbore chromatographic system was used to monitor changes in extracellular dopamine concentration in the striatum of the rat following administration of drugs that block catecholamine uptake. Analysis of 0.5 microliter of dialysate every 5 min showed dose-dependent elevations in extracellular dopamine following systemic administration of nomifensine (1 and 10 mg/kg), benztropine (5 and 25 mg/kg) and cocaine (3, 10 and 30 mg/kg). The order of potency in vivo was nomifensine greater than cocaine greater than benztropine. The short sampling interval allows accurate temporal profiles following pharmacological manipulations to be acquired.
使用微透析/小口径色谱系统监测大鼠纹状体中细胞外多巴胺浓度在给予阻断儿茶酚胺摄取药物后的变化。每5分钟分析0.5微升透析液显示，在全身给予诺米芬辛（1和10毫克/千克）、苯海索（5和25毫克/千克）和可卡因（3、10和30毫克/千克）后，细胞外多巴胺呈剂量依赖性升高。体内效力顺序为诺米芬辛大于可卡因大于苯海索。短采样间隔允许获取药理学操作后的准确时间曲线。
