Beran M, Zander A R, Andersson B S, McCredie K B
University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston, Department of Hematology.
Eur J Haematol. 1987 Aug;39(2):118-24. doi: 10.1111/j.1600-0609.1987.tb00740.x.
The hematopoietic capacity of bone marrow treated with 4-hydroperoxycyclophosphamide (4-HC) was studied using long-term suspension cultures. It was shown that, in the presence of a previously established marrow adherent cell layer (ACL), the 4-HC treated bone marrow, deprived of GM-CFC, generates GM-CFC in long-term suspension cultures in vitro. The kinetics of GM-CFC reappearance in in vitro cultures indicate their origin from more primitive progenitors rather than from surviving GM-CFC. In the presented modification, the suspension culture system is useful in evaluating the transplantation potential of bone marrow purged in vitro with chemotherapeutic or biologic agents.
利用长期悬浮培养法研究了经4-氢过氧环磷酰胺（4-HC）处理的骨髓的造血能力。结果表明，在预先建立的骨髓贴壁细胞层（ACL）存在的情况下，经4-HC处理且缺乏粒-巨噬细胞集落形成细胞（GM-CFC）的骨髓，在体外长期悬浮培养中能产生GM-CFC。GM-CFC在体外培养中重新出现的动力学表明它们起源于更原始的祖细胞，而非存活的GM-CFC。在本改良方法中，悬浮培养系统有助于评估经化疗或生物制剂体外净化的骨髓的移植潜力。
