Gordon M Y, Goldman J M, Gordon-Smith E C
Leuk Res. 1985;9(8):1017-21. doi: 10.1016/0145-2126(85)90072-4.
Despite its considerable toxicity to haemopoietic colony-forming cells, 4-hydroperoxycyclophosphamide (4-HC) has successfully been used to purge marrow of leukaemic cells before it is used to rescue patients from high-dose chemoradiotherapy. These conflicting observations indicate that haemopoietic progenitor cells that are not detected by the established colony-forming assays survive exposure to 4-HC and repopulate the marrow. The recent finding that murine spleen colony-forming cells (CFU-S) are resistant to 4-HC [Porcellini A, et al. (1983) Expl Hemat. 11 (suppl 14) 331 (abstract)] [14] also indicates that sensitivity to 4-HC can be used to distinguish primitive progenitor cells from committed progenitor cells. As part of a study on the nature of a population of blast colony-forming cells in human bone marrow, we tested their sensitivity to 4-HC to see whether they also are spared by the drug. We found that 4-HC had much less effect on the blast colony-forming cells than on the granulocyte-macrophage colony-forming cells (GM-CFC). This result suggests that the blast-colony-forming cells may be early human haemopoietic progenitor cells.
尽管4-氢过氧环磷酰胺（4-HC）对造血集落形成细胞具有相当大的毒性，但它已成功用于在高剂量放化疗后挽救患者之前清除骨髓中的白血病细胞。这些相互矛盾的观察结果表明，既定的集落形成试验未检测到的造血祖细胞在暴露于4-HC后仍能存活并重新填充骨髓。最近发现小鼠脾集落形成细胞（CFU-S）对4-HC具有抗性[Porcellini A等人（1983年），《实验血液学》。11（增刊14）331（摘要）][14]，这也表明对4-HC的敏感性可用于区分原始祖细胞和定向祖细胞。作为对人骨髓中一群原始细胞集落形成细胞性质研究的一部分，我们测试了它们对4-HC的敏感性，以了解它们是否也能免受该药物的影响。我们发现，4-HC对原始细胞集落形成细胞的影响比对粒细胞-巨噬细胞集落形成细胞（GM-CFC）的影响小得多。这一结果表明，原始细胞集落形成细胞可能是早期人类造血祖细胞。
