Tam P P, Chan W Y, Mao K R, Chiu T
Department of Anatomy, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T.
Fertil Steril. 1987 Nov;48(5):834-9. doi: 10.1016/s0015-0282(16)59540-5.
The embryotrophic property of patient's serum previously used for in vitro fertilization (IVF) was studied by culturing postimplantation organogenesis-stage mouse embryos in a medium containing equal parts of Dulbecco's modified Eagle's medium and the patient's serum. Results of embryo culture experiments were generally found to correlate well with the outcome of IVF. Serum of patients whose oocytes were fertilized and developed to cleavage-stage embryos scored highest for embryotrophic parameters such as morphologic score and protein content of the mouse embryo. There was no significant difference in terms of serum embryotrophic activity between patients who became pregnant after embryo transfer and those who did not. When mouse embryos were cultured in serum from cycles with poor IVF results, i.e., oocytes failed to be fertilized or fertilized oocytes failed to cleave or cleave abnormally, significantly retarded embryonic growth and a higher incidence of malformed embryos were observed. However, in two cases where IVF failed as a result of poor semen quality, the patient's serum was found to be supportive of mouse embryonic development.
通过在含有等量杜氏改良 Eagle 培养基和患者血清的培养基中培养植入后器官发生期小鼠胚胎，研究了先前用于体外受精（IVF）的患者血清的胚胎营养特性。胚胎培养实验结果通常与 IVF 结果密切相关。其卵母细胞受精并发育至卵裂期胚胎的患者血清，在诸如小鼠胚胎的形态学评分和蛋白质含量等胚胎营养参数方面得分最高。胚胎移植后怀孕的患者与未怀孕的患者在血清胚胎营养活性方面没有显著差异。当在 IVF 结果较差的周期的血清中培养小鼠胚胎时，即卵母细胞未受精或受精的卵母细胞未分裂或异常分裂，观察到胚胎生长明显迟缓且畸形胚胎的发生率更高。然而，在两例因精液质量差导致 IVF 失败的病例中，发现患者血清对小鼠胚胎发育有支持作用。
