Tan Lan-Pin, Chen Mei-Er
Department of Entomology, National Chung-Hsing University, Taichung City 40227, Taiwan.
Insects. 2023 Jan 11;14(1):70. doi: 10.3390/insects14010070.
Prip () cDNA was cloned (GenBank accession no. OK318454), and the encoded 276-amino-acid protein indicated the typical aquaporin structure, including six transmembrane regions and two NPA motifs. The developmental and tissue profiles of transcription were determined. was highly transcribed in female adults, followed by larvae, pupae, and male adults. The transcriptional expression levels of were significantly high in the ovary and hindgut (including cryptonephridial systems) compared with the foregut, testis, midgut, and Malpighian tubules. Knockdown of in female adults did not decrease fecundity, but significantly decreased the hatching rate of eggs laid by the females. The results suggest that functions in embryonic development, not in egg formation. In addition, the transcriptional expression level of was lower in the spinosad-resistant strain than in the susceptible one, and the resistant strain produced fewer progeny than the susceptible strain did. These studies support the functional role of in female reproduction. The absence of significant mortality reduction in the exposed to spinosad after RNAi suggests that other aquaporins that were not knocked down may exist for the excretion of metabolized pesticides.
克隆了Prip () cDNA（GenBank登录号：OK318454），编码的276个氨基酸的蛋白质显示出典型的水通道蛋白结构，包括六个跨膜区域和两个NPA基序。确定了转录的发育和组织谱。在成年雌性中高度转录，其次是幼虫、蛹和成年雄性。与前肠、睾丸、中肠和马氏管相比，在卵巢和后肠（包括隐肾系统）中 的转录表达水平显著较高。敲低成年雌性中的 不会降低繁殖力，但会显著降低雌性所产卵的孵化率。结果表明， 在胚胎发育中起作用，而不是在卵子形成中起作用。此外，抗多杀菌素品系中的 转录表达水平低于敏感品系，且抗药品系产生的后代比敏感品系少。这些研究支持了 在雌性繁殖中的功能作用。RNA干扰后暴露于多杀菌素的 中没有显著降低死亡率，这表明可能存在其他未被敲低的水通道蛋白用于代谢农药的排泄。
