Marine College, Shandong University, Weihai 264209, China.
School of Mechanical, Electrical and Information Engineering, Shandong University, Weihai 264209, China.
Mar Drugs. 2022 Dec 20;21(1):1. doi: 10.3390/md21010001.
The antioxidant effect of probiotics has been widely recognized across the world, which is of great significance in food, medicine, and aquaculture. There are abundant marine microbial resources in the ocean, which provide a new space for humans to explore new probiotics. Previously, we reported on the anti-infective effects of ML1206, a potential marine probiotic. The antioxidant activity of ML1206 in was studied in this paper. The study showed that ML1206 could improve the ability of nematodes to resist oxidative stress and effectively prolong their lifespan. The results confirmed that ML1206 could significantly increase the activities of CAT and GSH-PX, and reduce the accumulation of reactive oxygen species (ROS) in nematodes under oxidative stress conditions. In addition, ML1206 promoted DAF-16 transfer to the nucleus and upregulated the expression of , , and , which are downstream antioxidant-related genes of DAF-16. Furthermore, the expression of the SOD-3::GFP and HSP-16.2::GFP was significantly higher in the transgenic strains fed with ML1206 than that in the control group fed with OP50, with or without stress. In summary, these findings suggest that ML1206 is a novel marine probiotic with an antioxidant function that stimulates nematodes to improve their defense abilities against oxidative stress and prolong the lifespan by regulating the translocation of FOXO/DAF-16. Therefore, ML1206 may be explored as a potential dietary supplement in aquaculture and for anti-aging and antioxidant purposes.
益生菌的抗氧化作用已在全球范围内得到广泛认可，这在食品、医药和水产养殖领域具有重要意义。海洋中蕴藏着丰富的微生物资源，为人类探索新的益生菌提供了新的空间。此前，我们报道了一种潜在的海洋益生菌 ML1206 的抗感染作用。本文研究了 ML1206 在中的抗氧化活性。研究表明，ML1206 可以提高线虫抵抗氧化应激的能力，并有效延长其寿命。结果证实，ML1206 可显著提高 CAT 和 GSH-PX 的活性，并减少线虫在氧化应激条件下活性氧（ROS）的积累。此外，ML1206 促进了 DAF-16 向核内转移，并上调了抗氧化相关基因下游的 、 、 和 的表达。此外，在转 SOD-3::GFP 和 HSP-16.2::GFP 基因的菌株中，无论是否有应激，喂食 ML1206 的菌株的表达均明显高于喂食 OP50 的对照菌株。综上所述，这些发现表明，ML1206 是一种具有抗氧化功能的新型海洋益生菌，它通过调节 FOXO/DAF-16 的易位来刺激线虫提高其防御氧化应激的能力并延长寿命。因此，ML1206 可能被探索作为水产养殖中的潜在膳食补充剂，以及用于抗衰老和抗氧化目的。
