Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany.
BMC Complement Altern Med. 2019 Jul 8;19(1):164. doi: 10.1186/s12906-019-2578-5.
Caesalpinia mimosoides, a vegetable consumed in Thailand, has been reported to exhibit in vitro antioxidant properties. The in vivo antioxidant and anti-aging activities have not been investigated. The aim of this research was to study the antioxidant activity of C. mimosoides extracts in Caenorhabditis elegans, a widely used model organism in this context.
C. elegans were treated with C. mimosoides extracts in a various concentrations. To investigate the protective effects of the extract against oxidative stress, wild-type N2 were used to determine survival rate under oxidative stress and intracellular ROS. To study underlying mechanisms, the mutant strains with GFP reporter gene including TJ356, CF1553, EU1 and LD4 were used to study DAF-16, SOD-3, SKN-1 and GST-4 gene, respectively. Lifespan and aging pigment of the worms were also investigated.
A leaf extract of C. mimosoides improved resistance to oxidative stress and reduced intracellular ROS accumulation in nematodes. The antioxidant effects were mediated through the DAF-16/FOXO pathway and SOD-3 expression, whereas the expression of SKN-1 and GST-4 were not altered. The extract also prolonged lifespan and decreased aging pigments, while the body length and brood size of the worms were not affected by the extract, indicating low toxicity and excluding dietary restriction.
The results of this study establish the antioxidant activity of C. mimosoides extract in vivo and suggest its potential as a dietary supplement and alternative medicine to defend against oxidative stress and aging, which should be investigated in intervention studies.
在泰国食用的植物金凤花已被报道具有体外抗氧化特性。其体内抗氧化和抗衰老活性尚未被研究。本研究旨在研究金凤花提取物在秀丽隐杆线虫中的抗氧化活性，秀丽隐杆线虫是该领域广泛使用的模式生物。
用不同浓度的金凤花提取物处理秀丽隐杆线虫。为了研究提取物对氧化应激的保护作用，使用野生型 N2 确定在氧化应激和细胞内 ROS 下的存活率。为了研究潜在机制，使用带有 GFP 报告基因的突变株，包括 TJ356、CF1553、EU1 和 LD4，分别研究 DAF-16、SOD-3、SKN-1 和 GST-4 基因。还研究了蠕虫的寿命和衰老色素。
金凤花叶提取物提高了线虫对氧化应激的抵抗力并减少了细胞内 ROS 的积累。抗氧化作用是通过 DAF-16/FOXO 途径和 SOD-3 表达介导的，而 SKN-1 和 GST-4 的表达没有改变。提取物还延长了寿命并减少了衰老色素，而蠕虫的体长和产卵量不受提取物的影响，表明其毒性低且不存在饮食限制。
本研究确立了金凤花提取物在体内的抗氧化活性，并表明其作为一种膳食补充剂和替代药物具有防御氧化应激和衰老的潜力，这应该在干预研究中进行研究。
