Besis Athanasios, Romano Maria Pia, Serafeim Eleni, Avgenikou Anna, Kouras Athanasios, Lionetto Maria Giulia, Guascito Maria Rachele, De Bartolomeo Anna Rita, Giordano Maria Elena, Mangone Annarosa, Contini Daniele, Samara Constantini
Environmental Pollution Control Laboratory, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Department of Mathematics and Physics, University of Salento, 73100 Lecce, Italy.
Toxics. 2023 Jan 7;11(1):59. doi: 10.3390/toxics11010059.
Throughout the cold and the warm periods of 2020, chemical and toxicological characterization of the water-soluble fraction of size segregated particulate matter (PM) (<0.49, 0.49−0.95, 0.95−1.5, 1.5−3.0, 3.0−7.2 and >7.2 μm) was conducted in the urban agglomeration of Thessaloniki, northern Greece. Chemical analysis of the water-soluble PM fraction included water-soluble organic carbon (WSOC), humic-like substances (HULIS), and trace elements (V, Cr, Mn, Fe, Ni, Cu, Zn, As, Cd and Pb). The bulk (sum of all size fractions) concentrations of HULIS were 2.5 ± 0.5 and 1.2 ± 0.3 μg m−3, for the cold and warm sampling periods, respectively with highest values in the <0.49 μm particle size fraction. The total HULIS-C/WSOC ratio ranged from 17 to 26% for all sampling periods, confirming that HULIS are a significant part of WSOC. The most abundant water-soluble metals were Fe, Zn, Cu, and Mn. The oxidative PM activity was measured abiotically using the dithiothreitol (DTT) assay. In vitro cytotoxic responses were investigated using mitochondrial dehydrogenase (MTT). A significant positive correlation was found between OPmDTT, WSOC, HULIS and the MTT cytotoxicity of PM. Multiple Linear Regression (MLR) showed a good relationship between OPMDTT, HULIS and Cu.
在2020年的寒冷期和温暖期，对希腊北部塞萨洛尼基城市群中按粒径分类的颗粒物（PM）（<0.49、0.49−0.95、0.95−1.5、1.5−3.0、3.0−7.2和>7.2μm）的水溶性部分进行了化学和毒理学表征。水溶性PM部分的化学分析包括水溶性有机碳（WSOC）、类腐殖质（HULIS）和微量元素（V、Cr、Mn、Fe、Ni、Cu、Zn、As、Cd和Pb）。在寒冷和温暖采样期，HULIS的总体（所有粒径分数之和）浓度分别为2.5±0.5和1.2±0.3μg m−3，在<0.49μm粒径分数中最高。在所有采样期，HULIS-C/WSOC的总比值在17%至26%之间，证实HULIS是WSOC的重要组成部分。最丰富的水溶性金属是Fe、Zn、Cu和Mn。使用二硫苏糖醇（DTT）试验非生物地测量氧化PM活性。使用线粒体脱氢酶（MTT）研究体外细胞毒性反应。发现OPmDTT、WSOC、HULIS与PM的MTT细胞毒性之间存在显著正相关。多元线性回归（MLR）显示OPMDTT、HULIS和Cu之间存在良好关系。
