Chisu Valentina, Serra Elisa, Foxi Cipriano, Chessa Giovanna, Masala Giovanna
Department of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, Via Vienna, 2, 07100 Sassari, Italy.
Vet Sci. 2023 Jan 14;10(1):59. doi: 10.3390/vetsci10010059.
Piroplasmoses are tick-borne diseases caused by hemoprotozoan parasites of veterinary and public health significance. This study focuses on the molecular identification and characterization of species belonging to the genera in 152 blood samples, collected from 80 horses and 72 cattle from several farms in Sardinia, by targeting the 18S rRNA gene. The PCR results highlighted that 72% of the samples were positive for spp., with a rate of infection of 68% and 75% for the horses and cattle, respectively. Sequencing and the BLASTn analysis showed that the 18S rRNA generated in this study has 99-100% homology with the , , and strains isolated from different hosts worldwide. These findings improve the knowledge on and infections in domestic mammals and confirm the significant prevalence of piroplasmosis among subclinical and carrier animals throughout the island. Furthermore, the presence of , reported for the first time in the study area, expands the repertoire of pathogens already detected in Sardinia. Our results gather updates on the diversity and distribution of piroplasms in Sardinia and suggest the need to develop procedures to improve animal and public health safety.
梨形虫病是由具有兽医和公共卫生意义的血液原虫寄生虫引起的蜱传疾病。本研究通过靶向18S rRNA基因，对从撒丁岛几个农场的80匹马和72头牛采集的152份血液样本中属于该属的物种进行分子鉴定和特征分析。PCR结果显示，72%的样本对 spp.呈阳性，马和牛的感染率分别为68%和75%。测序和BLASTn分析表明，本研究中产生的18S rRNA与从世界各地不同宿主分离的 、 、 和 菌株具有99-100%的同源性。这些发现提高了对家养哺乳动物中 和 感染的认识，并证实了梨形虫病在全岛亚临床和携带动物中的显著流行率。此外，本研究首次报道的 的存在，扩大了撒丁岛已检测到的病原体种类。我们的结果收集了撒丁岛梨形虫多样性和分布的最新信息，并表明需要制定程序以改善动物和公共卫生安全。
