BW2013 protects mucosal integrity and modulates gut microbiota of mice with colitis.

This study explored the effects of (previously ) BW2013 on mucosal integrity and gut microbiota of mice with dextran sulfate sodium (DSS)-induced colitis. The results show that the clinical symptoms in DSS-modelled ulcerative colitis (UC) were improved by BW2013 via decreasing disease activity index scores and suppressing inflammatory cell infiltration. Furthermore, BW2013 decreased the levels of diamine oxidase activity, myeloperoxidase, and D-lactic acid. The mRNA expression of ZO-1, occludin, and claudin-1 was upregulated by BW2013, which also increased IL-10 and reduced TNF-α, IL-1β, and IL-6 in the colon. 16S rDNA sequencing showed that BW2013 enhanced α-diversity. BW2013 upregulated significantly the abundance of unidentfied , , , , and , which had an inhibitory effect on inflammation and a protective effect on the integrity of the mucosa. These results demonstrate that BW2013 alleviates DSS-modelled UC by protecting mucosal integrity and ameliorating the composition of gut microbiota.