Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing 100023, China.
Internal Trade Food Science and Technology (Beijing) Co., Ltd, Beijing 102209, China.
Can J Microbiol. 2023 Apr 1;69(4):158-169. doi: 10.1139/cjm-2022-0092. Epub 2023 Jan 20.
This study explored the effects of (previously ) BW2013 on mucosal integrity and gut microbiota of mice with dextran sulfate sodium (DSS)-induced colitis. The results show that the clinical symptoms in DSS-modelled ulcerative colitis (UC) were improved by BW2013 via decreasing disease activity index scores and suppressing inflammatory cell infiltration. Furthermore, BW2013 decreased the levels of diamine oxidase activity, myeloperoxidase, and D-lactic acid. The mRNA expression of ZO-1, occludin, and claudin-1 was upregulated by BW2013, which also increased IL-10 and reduced TNF-α, IL-1β, and IL-6 in the colon. 16S rDNA sequencing showed that BW2013 enhanced α-diversity. BW2013 upregulated significantly the abundance of unidentfied , , , , and , which had an inhibitory effect on inflammation and a protective effect on the integrity of the mucosa. These results demonstrate that BW2013 alleviates DSS-modelled UC by protecting mucosal integrity and ameliorating the composition of gut microbiota.
本研究探讨了 (先前 )BW2013 对葡聚糖硫酸钠 (DSS)诱导结肠炎小鼠黏膜完整性和肠道微生物群的影响。结果表明，BW2013 通过降低疾病活动指数评分和抑制炎症细胞浸润，改善 DSS 诱导的溃疡性结肠炎 (UC)的临床症状。此外，BW2013 降低了二胺氧化酶活性、髓过氧化物酶和 D-乳酸的水平。BW2013 上调了 ZO-1、occludin 和 claudin-1 的 mRNA 表达，同时增加了结肠中的 IL-10 并降低了 TNF-α、IL-1β 和 IL-6。16S rDNA 测序显示，BW2013 增强了 α-多样性。BW2013 显著上调了未鉴定的 、 、 、 、和 的丰度，它们对炎症具有抑制作用，对黏膜完整性具有保护作用。这些结果表明，BW2013 通过保护黏膜完整性和改善肠道微生物群组成来缓解 DSS 诱导的 UC。
