Department of Food Science and Technology, College of Agriculture and Environmental Sciences, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.
Department of Food Science and Technology, College of Agriculture and Environmental Sciences, University of California Davis, One Shields Avenue, Davis, CA 95616, USA; West Coast Metabolomics Center, Genome Center, University of California Davis, One Shields Avenue, Davis, CA 95616, USA; Center for Neuroscience, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Feb 1;1216:123585. doi: 10.1016/j.jchromb.2022.123585. Epub 2022 Dec 26.
Carotenoid pigmentation in salmon may interfere with the accuracy of antibiotic analysis with ultra-high pressure liquid chromatography coupled to tandem spectrometry (UPLC-MS/MS) by causing matrix effects or affecting the recovery of compounds during extraction. In the present study, we used both pigmented and non-pigmented salmon to understand the role pigments play on antibiotic analysis, and tested whether clean-up of the extract with dispersive solid phase extraction (dSPE) or hydrophilic-lipophilic balance (HLB) SPE clean-up reduces matrix effects. Thirty antibiotics and their respective class-specific surrogate standards were measured in Sockeye (pigmented), King (pigmented) and Ivory King (non-pigmented) salmon extracted using the QUEChERS method, or a modified QUEChERS method involving dSPE or HLB SPE clean-up (for Sockeye salmon only). Significant matrix effects and lower percent recoveries of spiked antibiotics were observed in pigmented versus non-pigmented salmon extracted with the QUEChERS method. Dispersive SPE clean-up did not improve extraction recoveries or matrix effects. However, SPE clean-up with HLB columns improved matrix effects for several antibiotics but reduced the percent recovery to < 30%. Across all types of salmon analyzed, the accuracy of quantitation was minimally impacted, likely due to similar behavior of the surrogate standards tagged to each antibiotic class during extraction. Our results demonstrate that carotenoids in salmon are associated with significant matrix effects and low extraction recoveries, but do not impact accuracy.
鲑鱼中的类胡萝卜素色素可能会通过基质效应或影响提取过程中化合物的回收率，干扰超高效液相色谱串联质谱法（UPLC-MS/MS）对抗生素分析的准确性。在本研究中，我们使用有色和无色鲑鱼来了解色素在抗生素分析中的作用，并测试提取物的净化是否通过分散固相萃取（dSPE）或亲水亲脂平衡（HLB）SPE 净化可以减少基质效应。使用 QuEChERS 方法或涉及 dSPE 或 HLB SPE 净化（仅适用于鲑鱼）的改良 QuEChERS 方法从红鲑（有色）、国王鲑（有色）和象牙国王鲑（无色）中提取 30 种抗生素及其各自的类特异性替代标准品，并进行测量。与使用 QuEChERS 方法从无色鲑鱼中提取的抗生素相比，有色鲑鱼中观察到显著的基质效应和更低的加标抗生素回收率。分散 SPE 净化不能提高提取回收率或基质效应。然而，HLB 柱 SPE 净化可改善几种抗生素的基质效应，但回收率降低至<30%。在分析的所有类型的鲑鱼中，定量的准确性受影响最小，这可能是由于在提取过程中与每个抗生素类别的替代标准品具有相似的行为。我们的结果表明，鲑鱼中的类胡萝卜素与显著的基质效应和低提取回收率有关，但不会影响准确性。
