Milojkov Dušan V, Radosavljević-Mihajlović Ana S, Stanić Vojislav Dj, Nastasijević Branislav J, Radotić Ksenija, Janković-Častvan Ivona, Živković-Radovanović Vukosava
Institute for Technology of Nuclear and Other Mineral Raw Materials, Franchet d'Esperey 86, P.O. Box 390, 11000 Belgrade, Serbia.
Institute for Technology of Nuclear and Other Mineral Raw Materials, Franchet d'Esperey 86, P.O. Box 390, 11000 Belgrade, Serbia.
J Photochem Photobiol B. 2023 Feb;239:112649. doi: 10.1016/j.jphotobiol.2023.112649. Epub 2023 Jan 14.
Nanomaterials based on metal-doped fluorapatite (FAP) have attracted considerable interest as potential next-generation antimicrobial agents. In this study, Cu-doped FAP nanocrystals have been successfully synthesized by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible properties have been investigated. XRD, FTIR, FESEM, and N adsorption-desorption studies indicate the formation of single-phase FAP mesoporous nanopowders, composed of rod-like particles. TEM images confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis shows that the Cu ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure. Fluorescence spectroscopy accompanied by MCR-ALS method confirms substitution of Cu ions in FAP crystal lattice with extracting additional d-d band transition at green color from FAP broadband self-activated luminescence in violet-blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu content have strong bacteriostatic action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of Aspergillus niger species. Cu-doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu and F ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu-doped FAP samples were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad-spectrum antimicrobial agents for various biomedical and health care preparations.
基于金属掺杂氟磷灰石（FAP）的纳米材料作为潜在的下一代抗菌剂引起了广泛关注。在本研究中，通过室温中和法成功合成了铜掺杂的FAP纳米晶体。对其结构、光学、抗菌和血液相容性进行了研究。XRD、FTIR、FESEM和N吸附-脱附研究表明形成了由棒状颗粒组成的单相FAP介孔纳米粉末。TEM图像证实形成了长度为60nm、宽度约为18nm的纳米棒。Rietveld分析表明，铜离子优先取代六方氟磷灰石晶体结构中的Ca2（6h）位点。荧光光谱结合MCR-ALS方法证实了FAP晶格中铜离子的取代，从FAP的紫蓝色宽带自激活发光中提取出额外的绿色d-d带跃迁。对金黄色葡萄球菌、大肠杆菌和溶壁微球菌进行的抗菌研究表明，铜含量最高的FAP纳米粉末对含有营养物质的培养基中的金黄色葡萄球菌菌株具有很强的抑菌作用。此外，与纯FAP相比，该样品对所有三种菌种的细菌数量相对减少率都很高，在大多数情况下>90%。还观察到了抑菌作用，通过减缓黑曲霉、黄曲霉和罗克福特青霉真菌的菌丝生长以及减少黑曲霉菌种的孢子形成来实现。铜掺杂的FAP纳米晶体显示出铜离子和氟离子的协同抗菌作用。关于潜在的生物医学应用，铜掺杂FAP样品的溶血率低于5%。所得结果指出了合成纳米晶体作为各种生物医学和医疗保健制剂的广谱抗菌剂的可能用途。
