Toepel Jörg, Karande Rohan, Klähn Stephan, Bühler Bruno
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.
Research and Transfer Center for bioactive Matter b-ACTmatter, University of Leipzig, Germany.
Curr Opin Biotechnol. 2023 Apr;80:102892. doi: 10.1016/j.copbio.2023.102892. Epub 2023 Jan 18.
Cyanobacteria as phototrophic microorganisms bear great potential to produce chemicals from sustainable resources such as light and CO. Most studies focus on either strain engineering or tackling metabolic constraints. Recently gained knowledge on internal electron and carbon fluxes and their regulation provides new opportunities to efficiently channel cellular resources toward product formation. Concomitantly, novel photobioreactor concepts are developed to ensure sufficient light supply. This review summarizes the newest developments in the field of cyanobacterial engineering to finally establish photosynthesis-based production processes. A holistic approach tackling genetic, metabolic, and biochemical engineering in parallel is considered essential to turn their application into an ecoefficient and economically feasible option for a future green bioeconomy.
蓝细菌作为光合微生物，具有利用光和二氧化碳等可持续资源生产化学品的巨大潜力。大多数研究要么集中在菌株工程，要么致力于解决代谢限制。最近关于内部电子和碳通量及其调控的知识为有效地将细胞资源导向产物形成提供了新机会。与此同时，新的光生物反应器概念也在不断发展，以确保充足的光照供应。本综述总结了蓝细菌工程领域的最新进展，以期最终建立基于光合作用的生产工艺。同时应对基因工程、代谢工程和生化工程的整体方法，被认为是将其应用转化为未来绿色生物经济中生态高效且经济可行选择的关键。
