Li Si, Shang Xiao-Jing, Luo Qi-Xing, Yan Qian, Hou Rui
College of Forestry, Guizhou University, Guiyang 550025, China.
FEMS Microbiol Ecol. 2023 Jan 24;99(2). doi: 10.1093/femsec/fiad008.
Blueberry is a shallow root plant in which the absorption of nutrients is inefficient, resulting in slow growth under artificial cultivation conditions. Endophytes play an important role in promoting plant growth; however, the effects of Trichoderma spp. and dark septate endophytes (DSEs) on host plant growth and soil microorganisms are still debatable. We isolated two endophytic fungal species, Trichoderma koningiopsis (TK) and a DSE (Amesia nigricolor; AN), from blueberry roots, which can solubilize insoluble phosphorus and produce amylase and cellulase to promote plant growth. We found that under dual inoculation, the colonization rate and colonization intensity of TK were higher than they were under single inoculation with TK, while the colonization rate and colonization intensity of AN were lower under dual inoculation than under single inoculation with AN. The plant nutrients, root activity, available potassium, and parts of soil phosphatase activities were highest under dual inoculation. TK inoculation resulted in the highest diversity and richness in the soil fungi and bacteria, followed by dual inoculation. The abundance of Ascomycota, Acidobacteriae, Firmicutes, and Actinobacteriota increased significantly, resulting in Trichoderma and Vicinamibacteria inoculated with TK, Chaetomium and Alicyclobacillales inoculated with AN, and Hypocreales and Burkholderiaceae with dual inoculation enriched in the soil.
蓝莓是一种浅根植物，其养分吸收效率低下，导致在人工栽培条件下生长缓慢。内生菌在促进植物生长方面发挥着重要作用；然而，木霉菌属和深色有隔内生菌（DSE）对寄主植物生长和土壤微生物的影响仍存在争议。我们从蓝莓根系中分离出两种内生真菌，即康氏木霉（TK）和一种DSE（黑阿美菌；AN），它们可以溶解难溶性磷并产生淀粉酶和纤维素酶以促进植物生长。我们发现，在双重接种下，TK的定殖率和定殖强度高于单一接种TK时，而双重接种下AN的定殖率和定殖强度低于单一接种AN时。在双重接种下，植物养分、根系活力、速效钾和部分土壤磷酸酶活性最高。接种TK导致土壤真菌和细菌的多样性和丰富度最高，其次是双重接种。子囊菌门、酸杆菌门、厚壁菌门和放线菌门的丰度显著增加，导致接种TK的木霉属和邻单胞菌属、接种AN的毛壳菌属和 Alicyclobacillales以及双重接种的肉座菌目和伯克霍尔德氏菌科在土壤中富集。
