College of Forestry, Guizhou University, Guiyang, 550025, People's Republic of China.
J Microbiol. 2023 Sep;61(9):837-851. doi: 10.1007/s12275-023-00080-2. Epub 2023 Oct 5.
Dark septate endophytes (DSEs) are widely distributed and improve plant growth. DSEs secrete large amounts of enzymes to mineralize insoluble phosphorus in soil and convert it into soluble phosphorus, promoting plant uptake of phosphorus. However, the effects of DSEs with phosphate-solubilizing ability on host plants need further study. In this study, phosphorus-dissolving DSEs were screened for growth-promoting effects. We isolated, identified and characterized three DSE species (Thozetella neonivea, Pezicula ericae and Hyaloscyphaceae sp.) showing phosphate-solubilizing ability. The impact of single, dual or triple inoculation of DSEs on blueberry plant characteristics was studied. Their effects on colonization intensity, seedling biomass, nutrients in plants and soil, and activities of plant resistance enzymes and soil enzymes were markedly upregulated relative to the control (P < 0.05). The available phosphorus and acid phosphatase levels in different combinations were significantly increased. These findings indicate that the application of the three DSEs may be valuable in facilitating the cultivation of blueberry with a higher biomass and improved plant quality.
深色有隔内生真菌（DSE）广泛分布，并能促进植物生长。DSE 分泌大量的酶来矿化土壤中不溶性磷，并将其转化为可溶性磷，从而促进植物对磷的吸收。然而，具有解磷能力的 DSE 对宿主植物的影响需要进一步研究。在本研究中，筛选了具有促生长作用的解磷 DSE。我们分离、鉴定和表征了三种具有解磷能力的 DSE 物种（Neivea neonivea、Erica pezicula 和 Hyaloscyphaceae sp.）。研究了 DSE 单一、双重或三重接种对蓝莓植物特性的影响。与对照相比（P < 0.05），它们对定植强度、幼苗生物量、植物养分和土壤养分、植物抗性酶和土壤酶活性的影响明显上调。不同组合的有效磷和酸性磷酸酶水平显著增加。这些发现表明，三种 DSE 的应用可能有助于促进具有更高生物量和改善植物品质的蓝莓栽培。
