Clinical Biochemistry and Molecular Diagnostics Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt.
Pathology Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt.
Biomolecules. 2022 Dec 20;13(1):3. doi: 10.3390/biom13010003.
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Interestingly, lncRNA-H19 acts independently in HCC and influences miR-675 expressions. We aimed to assess the potential utility of tissue lncRNA-H19 versus miR-675 expressions as a non-invasive biomarker for HCC diagnosis and prognosis in Egyptian patients. Ninety-one HCC patients and 91 controls included in this study were investigated for expression of lncRNA-H19 and miR675 using RT-qPCR. Our results showed that the expression of lncRNA-H19 and microRNA-675 were higher in patients than in controls ( < 0.001 for both). Additionally, lncRNA-H19 expression was higher in tumorous than in non-tumorous tissue ( < 0.001). Linear regression revealed that miR-675 expression was a significantly higher positive predictor than lncRNA-H19 for tumor size, pathologic grade, and AFP level; similarly, for cyclin D1 and VEGF protein expression. By using the ROC curve, the sensitivity of miR-675 was higher than lncRNA-H19 for discriminating HCC from controls (95-89%, respectively) and the sensitivity of lncRNA-H19 was higher in tumorous than in non-tumorous tissues (76%). The high expressions of both were associated with low OS ( < 0.001, 0.001, respectively). Oncofetal H19-derived miR-675 expression could be considered a potential noninvasive diagnostic and prognostic biomarker, outstanding the performance of the expression of tissue lncRNA-H19 for HCC.
肝细胞癌 (HCC) 是全球最常见的癌症之一。有趣的是，lncRNA-H19 在 HCC 中独立发挥作用，并影响 miR-675 的表达。我们旨在评估组织 lncRNA-H19 与 miR-675 表达作为埃及患者 HCC 诊断和预后的非侵入性生物标志物的潜在用途。本研究纳入了 91 例 HCC 患者和 91 例对照，采用 RT-qPCR 检测 lncRNA-H19 和 miR675 的表达。我们的结果表明，lncRNA-H19 和 microRNA-675 的表达在患者中高于对照组（两者均 < 0.001）。此外，lncRNA-H19 的表达在肿瘤组织中高于非肿瘤组织（<0.001）。线性回归显示，miR-675 的表达是肿瘤大小、病理分级和 AFP 水平的显著更高的阳性预测因子；同样，对于 cyclin D1 和 VEGF 蛋白表达也是如此。通过使用 ROC 曲线，miR-675 对 HCC 与对照组的鉴别敏感性高于 lncRNA-H19（分别为 95-89%），而 lncRNA-H19 在肿瘤组织中的敏感性高于非肿瘤组织（76%）。两者的高表达均与低 OS 相关（分别为 < 0.001、0.001）。癌胚基因 H19 衍生的 miR-675 表达可被认为是一种有潜力的非侵入性诊断和预后生物标志物，突出了组织 lncRNA-H19 对 HCC 的表达性能。
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