与细胞性基质血管成分相比，组织性基质血管成分治疗肛门括约肌失禁的效果。
The Effect of Tissue Stromal Vascular Fraction as Compared to Cellular Stromal Vascular Fraction to Treat Anal Sphincter Incontinence.
作者信息
Chen Wenbin, He Zijian, Li Shuyu, Wu Zixin, Tan Jin, Yang Weifeng, Li Guanwei, Pan Xiaoling, Liu Yuying, Lyu Feng-Juan, Li Wanglin
机构信息
Department of Colorectal and Anal Surgery, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510641, China.
School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510641, China.
出版信息
Bioengineering (Basel). 2022 Dec 26;10(1):32. doi: 10.3390/bioengineering10010032.
BACKGROUND
The long-term prognosis of current treatments for anal sphincter incontinence (ASI) is poor. Here, we explored the efficacy of tissue adipose stromal vascular fraction SVF (tSVF) on ASI and compared it to that of cellular SVF (cSVF). We then investigated possible mechanisms.
METHODS
Rat cSVF and tSVF were isolated and labeled with DIL. One day after modeling, three groups received phosphate-buffered saline (PBS), cSVF, tSVF, respectively. The control group received nil modeling nor any treatments. The effect was assessed by function test for anal pressure and electromyography, and staining for fiber content, proliferation and differentiation at day 5 and day 10.
RESULTS
cSVF injection resulted in faster healing than tSVF. The cSVF group showed significant improvement on anal pressure on day 10. For the electromyography test, cSVF showed significant improvement for the frequencies on day 10, and for the peak values on both time points, while tSVF showed significant improvement for the peak values on day 10. The two SVF both alleviated fibrosis. Immunofluorescence tracing identified differentiation of some injected cells towards myosatellite cells and smooth muscle cells in both SVF groups. For all the tests, the tSVF group tends to have similar or lower effects than the cSVF group with no significant difference.
CONCLUSION
cSVF and tSVF are both safe and effective in treating ASI, while the effect of cSVF is slighter higher than tSVF.
背景
目前肛门括约肌失禁（ASI）治疗方法的长期预后较差。在此，我们探讨了组织脂肪基质血管成分SVF（tSVF）对ASI的疗效，并将其与细胞SVF（cSVF）的疗效进行比较。然后我们研究了可能的机制。
方法
分离大鼠cSVF和tSVF并用DIL标记。建模一天后，三组分别接受磷酸盐缓冲盐水（PBS）、cSVF、tSVF。对照组既不建模也不接受任何治疗。在第5天和第10天通过肛门压力功能测试和肌电图评估效果，并对纤维含量、增殖和分化进行染色。
结果
注射cSVF比tSVF愈合更快。cSVF组在第10天肛门压力有显著改善。对于肌电图测试，cSVF在第10天频率有显著改善，在两个时间点峰值均有显著改善，而tSVF在第10天峰值有显著改善。两种SVF均减轻了纤维化。免疫荧光追踪确定在两个SVF组中一些注射细胞向肌卫星细胞和平滑肌细胞分化。对于所有测试，tSVF组的效果往往与cSVF组相似或更低，无显著差异。
结论
cSVF和tSVF治疗ASI均安全有效，而cSVF的效果略高于tSVF。
Zotero 插件