Department of Internal Medicine, Division of Infectious Diseases, Wan Fang Hospital, Taipei Medical University, No.111, Sec. 3, Xinglong Rd., Wenshan Dist., Taipei 11600, Taiwan.
Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.
Biosensors (Basel). 2022 Dec 30;13(1):60. doi: 10.3390/bios13010060.
The early diagnosis of acute myocardial infarction is difficult in patients with nondiagnostic characteristics. Acute myocardial infarction with chest pain is associated with increased mortality. This study developed a portable test kit based on cholesteric liquid crystals (CLCs) for the rapid detection of AMI through eye observation at home. The test kit was established on dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride-coated substrates covered by a CLC-binding antibody. Cardiac troponin I (cTnI) is a major biomarker of myocardial cellular injury in human blood. The data showed that the concentration of cTnI was related to light transmittance in a positive way. The proposed CLC test kit can be operated with a smartphone; therefore, it has high potential for use as a point-of-care device for home testing. Moreover, the CLC test kit is an effective and innovative device for the rapid testing of acute myocardial infarction-related diseases through eye observation, spectrometer, or even smartphone applications.
对于不具有典型特征的急性心肌梗死（AMI）患者，早期诊断较为困难。胸痛是急性心肌梗死的主要症状，与死亡率增加相关。本研究基于胆甾相液晶（CLC）开发了一种便携式检测试剂盒，可通过在家中眼部观察进行快速检测。该试剂盒是在涂有二甲基十八烷基[3-（三甲氧基硅基）丙基]氯化铵的基底上建立的，基底上覆盖有与 CLC 结合的抗体。心肌肌钙蛋白 I（cTnI）是人类血液中心肌细胞损伤的主要生物标志物。结果表明，cTnI 的浓度与光透过率呈正相关。所提出的 CLC 检测试剂盒可以与智能手机配合使用；因此，它具有作为家庭检测即时护理设备的巨大潜力。此外，CLC 检测试剂盒是一种通过眼部观察、分光光度计甚至智能手机应用程序进行快速检测与急性心肌梗死相关疾病的有效且创新的设备。
