Univ. de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d'Infection et d'Immunité de Lille, CHU Lille, Surgical Critical Care, Department of Anesthesiology and Critical Care, F-59000 Lille, France.
Biothelis, F-59000 Lille, France.
Cells. 2023 Jan 7;12(2):257. doi: 10.3390/cells12020257.
Acute respiratory distress syndrome (ARDS) is a life-threatening condition resulting from acute pulmonary inflammation. However, no specific treatment for ARDS has yet been developed. Previous findings suggest that lung injuries related to ARDS could be regulated by endocan (Esm-1). The aim of this study was to evaluate the potential efficiency of endocan in the treatment of ARDS.
We first compared the features of acute pulmonary inflammation and the severity of hypoxemia in a tracheal LPS-induced acute lung injury (ALI) model performed in knockout () and wild type (WT) littermate C57Bl/6 mice. Next, we assessed the effects of a continuous infusion of glycosylated murine endocan in our ALI model in mice.
In our ALI model, we report higher alveolar leukocytes ( < 0.001), neutrophils ( < 0.001), and MPO ( < 0.001), and lower blood oxygenation ( < 0.001) in mice compared to WT mice. Continuous delivery of glycosylated murine endocan after LPS-induced ALI resulted in decreased alveolar leukocytes ( = 0.012) and neutrophils ( = 0.012), higher blood oxygenation levels ( < 0.001), and reduced histological lung injury ( = 0.04), compared to mice treated with PBS.
Endocan appears to be an effective treatment in an ARDS-like model in C57Bl/6 mice.
急性呼吸窘迫综合征（ARDS）是一种由急性肺炎症引起的危及生命的病症。然而，目前尚未开发出针对 ARDS 的特定治疗方法。先前的研究结果表明，与 ARDS 相关的肺损伤可能受内参（Esm-1）调节。本研究旨在评估内参在 ARDS 治疗中的潜在疗效。
我们首先比较了气管 LPS 诱导的急性肺损伤（ALI）模型中敲除（）和野生型（WT）同窝 C57Bl/6 小鼠的急性肺炎症特征和低氧血症严重程度。接下来，我们评估了在我们的 ALI 模型中连续输注糖基化鼠内参对小鼠的影响。
在我们的 ALI 模型中，我们报告说与 WT 小鼠相比， 小鼠的肺泡白细胞（<0.001）、中性粒细胞（<0.001）和髓过氧化物酶（MPO）（<0.001）更高，而血氧水平（<0.001）更低。在 LPS 诱导的 ALI 后连续给予糖基化鼠内参可导致肺泡白细胞（=0.012）和中性粒细胞（=0.012）减少，血氧水平升高（<0.001），以及组织学肺损伤减少（=0.04），与用 PBS 治疗的小鼠相比。
内参似乎是 C57Bl/6 小鼠 ARDS 样模型中的一种有效治疗方法。
