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酶联免疫吸附测定（ELISA）（原理、技术及应用）

The enzyme-linked immunosorbent assay (ELISA) (theory, technique and applications).

作者信息

Voller A

出版信息

Ric Clin Lab. 1978 Oct-Dec;8(4):289-98.

PMID:366726
Abstract

Enzyme-linked immunosorbent assays (ELISA) are useful for measuring levels on antigens and antibodies. Both competitive and sandwich methods can be used. These assays are analogous to radioimmunoassays in format and have similar sensitivity. However, results can sometimes by read visually and even for objective readings only simple equipment is needed. ELISA has been used to measure hormones and other protein antigens and has also been used to assay antibody levels.

摘要

酶联免疫吸附测定（ELISA）可用于测量抗原和抗体水平。竞争性和夹心方法均可使用。这些测定在形式上类似于放射免疫测定，并且具有相似的灵敏度。然而，结果有时可以通过肉眼读取，甚至对于客观读数仅需要简单的设备。ELISA已用于测量激素和其他蛋白质抗原，也已用于测定抗体水平。

相似文献

1
The enzyme-linked immunosorbent assay (ELISA) (theory, technique and applications).酶联免疫吸附测定（ELISA）（原理、技术及应用）
Ric Clin Lab. 1978 Oct-Dec;8(4):289-98.
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The use of the enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of specific antibody from cell cultures.使用酶联免疫吸附测定法（ELISA）检测和定量细胞培养物中的特异性抗体。
Immunology. 1979 May;37(1):45-52.
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ELISA: a tool for the clinical microbiologist.酶联免疫吸附测定：临床微生物学家的一种工具。
Am J Med Technol. 1981 Nov;47(11):905-8.
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Enzyme-linked immunosorbent assay (ELISA) for streptokinase antibodies.链激酶抗体的酶联免疫吸附测定（ELISA）
Diagn Immunol. 1983;1(2):64-7.
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Application of enzyme-linked immunosorbent assay (ELISA) in diagnosis of allergic bronchopulmonary aspergillosis.酶联免疫吸附测定（ELISA）在变应性支气管肺曲霉病诊断中的应用。
J Lab Clin Med. 1982 Feb;99(2):288-93.
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Enzyme-linked immunosorbent assay (ELISA): a practical tool for rapid diagnosis of viruses and other infectious agents.酶联免疫吸附测定（ELISA）：一种用于快速诊断病毒及其他感染因子的实用工具。
Yale J Biol Med. 1980 Jan-Feb;53(1):85-92.
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Enzyme-linked immunosorbent assay for the detection of toxoplasmosis in swine: interpreting assay results and comparing with other serologic tests.用于检测猪弓形虫病的酶联免疫吸附测定：分析测定结果并与其他血清学检测方法进行比较
Am J Vet Res. 1984 Sep;45(9):1719-25.
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Rapid enzyme-linked immunosorbent assay for detecting antibodies to canine parvovirus.用于检测犬细小病毒抗体的快速酶联免疫吸附测定
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A new technique of heterogenous enzyme-linked immunosorbent assay, stick-ELISA. II. Application of stick-ELISA to antigens of various infective agents.一种新的异源酶联免疫吸附测定技术——棒式酶联免疫吸附测定法。II. 棒式酶联免疫吸附测定法在各种感染因子抗原检测中的应用。
Zentralbl Bakteriol Orig A. 1978 Jan;240(1):118-22.
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[Development and use of radio- and enzyme immunoassays in microbiological and immunological diagnosis. II. Comparative studies on Toxoplasma antibody determination with ELISA, RIA and other serological technics].[放射免疫测定和酶免疫测定在微生物学和免疫学诊断中的开发与应用。II. 酶联免疫吸附测定法、放射免疫测定法及其他血清学技术在弓形虫抗体检测中的比较研究]
Z Gesamte Hyg. 1982 Jun;28(6):375-7.

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