The emergence of tigecycline-resistant bacteria in agri-food chains poses a public health concern. Recently, plasmid-mediated (X4) was found to be resistant to tigecycline. However, genome differences between (X4)-positive of human and pork origins are still under-investigated. In this study, 53 pork samples were collected from markets in Jiangsu, China, and 23 (X4)-positive isolates were identified and shown to confer resistance to multiple antibiotics, including tigecycline. (X4)-positive isolates were mainly distributed in (n = 22), followed by (n = 1). More than half of the (X4) genes were able to be successfully transferred into C600. We downloaded all (X4)-positive isolates from humans and pork found in China from the NCBI database. A total of 42 known STs were identified, of which ST10 was the dominant ST. The number of ARGs and plasmid replicons carried by of human origin were not significantly different from those carried by of pork origin. However, the numbers of insertion sequences and virulence genes carried by of human origin were significantly higher than those carried by of pork origin. In addition to , we analyzed all 23 (X4)-positive strains currently reported. We found that these (X4)-positive were mainly distributed in China and had no dominant STs. This study systematically investigated the (X4)-positive isolates, emphasizing the importance of the continuous surveillance of (X4) in pork.