巴西和美国羊突变体的慢病毒易感性

Lentivirus Susceptibility in Brazilian and US Sheep with Mutations.

机构信息

Faculdade de Agronomia e Medicina Veterinária, Instituto Central de Ciências, Campus Darcy Ribeiro, Universidade de Brasília, Asa Norte, Brasilia 70910-900, DF, Brazil.

Embrapa Recursos Genéticos e Biotecnologia, Final W5 Norte, Brasilia 70770-917, DF, Brazil.

出版信息

Genes (Basel). 2022 Dec 26;14(1):70. doi: 10.3390/genes14010070.

DOI:10.3390/genes14010070

PMID:36672811

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9858560/

Abstract

Small ruminant lentiviruses (SRLVs) affect sheep and goats worldwide. The major gene related to SRLV infections is the Transmembrane Protein Gene 154 (). We estimated the haplotype frequencies of in the USA (USDA-ARS) and Brazil (Embrapa) Gene Banks by using two different SNP genotyping methodologies, Fluidigm and KASP. We also genotyped the ss748775100 deletion variant in Brazilian flocks. A total of 1040 blood samples and 112 semen samples from 15 Brazilian breeds were genotyped with Fluidigm for the SNP ss748775100 and 12 SNPs. A total of 484 blood samples from the Santa Inês breed and 188 semen samples from 14 North American sheep breeds were genotyped with KASP for 6 SNPs. All the Brazilian samples had the "I/I" genotype for the ss748775100 mutation. There were 25 haplotypes distributed across the Brazilian breeds, and 4 haplotypes in the US breeds. Haplotypes associated with susceptibility were present in almost all breeds, which suggests that genetic testing can help to improve herd health and productivity by selecting non-susceptible animals as founders of the next generations. Fluidigm and KASP are reliable assays when compared with Beadchip arrays. Further studies are necessary to understand the unknown role of mutations, host-pathogen interaction and new genes associated with the clinical condition.

摘要

小反刍兽瘟病毒（SRLV）在全球范围内影响绵羊和山羊。与 SRLV 感染相关的主要基因是跨膜蛋白基因 154（）。我们使用两种不同的 SNP 基因分型方法——Fluidigm 和 KASP——估计了美国（USDA-ARS）和巴西（Embrapa）基因库中的单倍型频率。我们还对巴西羊群中的 ss748775100 缺失变异进行了基因分型。我们使用 Fluidigm 对 15 个巴西品种的 1040 份血液样本和 112 份精液样本进行了 ss748775100 位点 SNP 和 12 个 SNP 的基因分型。我们使用 KASP 对圣塔因尼斯品种的 484 份血液样本和 14 个北美绵羊品种的 188 份精液样本进行了 6 个 SNP 的基因分型。所有巴西样本的 ss748775100 突变均为“II”基因型。在巴西品种中分布着 25 种单倍型，而美国品种中则有 4 种单倍型。与易感性相关的单倍型几乎存在于所有品种中，这表明通过选择不易感的动物作为下一代的基础，基因检测可以帮助改善畜群的健康和生产力。Fluidigm 和 KASP 与 Beadchip 阵列相比是可靠的检测方法。还需要进一步研究来了解突变、宿主-病原体相互作用和与临床状况相关的新基因的未知作用。