College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Genes (Basel). 2022 Dec 28;14(1):90. doi: 10.3390/genes14010090.
Fibronectin type III domain-containing protein 5 (FNDC5) plays an important role in fat deposition, which can be cut to form Irisin to promote fat thermogenesis, resulting in a decrease in fat content. However, the mechanism of FNDC5 related to fat deposition in pigs is still unclear. In this research, we studied the expression of FNDC5 on different adiposes and its function in the adipogenic differentiation of primary preadipocytes in Mashen pigs. The expression pattern of was detected by qRT-PCR and Western blotting in Mashen pigs. overexpression and interference vectors were constructed and transfected into porcine primary preadipocytes by lentivirus. Then, the expression of key adipogenic genes was detected by qRT-PCR and the content of lipid droplets was detected by Oil Red O staining. The results showed that the expression of in abdominal fat was higher than that in back subcutaneous fat in Mashen pigs, whereas the expression in back subcutaneous fat of Mashen pigs was significantly higher than that of Large White pigs. In vitro, FNDC5 promoted the adipogenic differentiation of primary preadipocytes of Mashen pigs and upregulated the expression of genes related to adipogenesis, but did not activate the extracellular signal-regulated kinase (ERK) signaling pathway. This study can provide a theoretical basis for FNDC5 in adipogenic differentiation in pigs.
纤连蛋白结构域蛋白 5（FNDC5）在脂肪沉积中发挥重要作用，其可被切割形成鸢尾素，促进脂肪产热，导致脂肪含量降低。然而，FNDC5 与猪脂肪沉积相关的机制尚不清楚。本研究旨在探讨 Mashen 猪不同脂肪组织中 FNDC5 的表达及其对原代前体脂肪细胞成脂分化的影响。采用 qRT-PCR 和 Western blot 检测 Mashen 猪中 FNDC5 的表达模式。构建 FNDC5 过表达和干扰载体，通过慢病毒转染猪原代前体脂肪细胞。然后，通过 qRT-PCR 检测关键成脂基因的表达，用油红 O 染色检测脂滴含量。结果表明，Mashen 猪腹部脂肪中的 FNDC5 表达高于背部皮下脂肪，而 Mashen 猪背部皮下脂肪中的 FNDC5 表达明显高于长白猪。体外实验表明，FNDC5 促进了 Mashen 猪原代前体脂肪细胞的成脂分化，并上调了与成脂相关的基因表达，但未激活细胞外信号调节激酶（ERK）信号通路。本研究可为 FNDC5 在猪成脂分化中的作用提供理论依据。
