College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, China.
BMC Genomics. 2019 Oct 15;20(1):743. doi: 10.1186/s12864-019-6116-0.
The distribution and deposition of fat tissue in different parts of the body are the key factors affecting the carcass quality and meat flavour of chickens. Intramuscular fat (IMF) content is an important factor associated with meat quality, while abdominal fat (AbF) is regarded as one of the main factors affecting poultry slaughter efficiency. To investigate the differentially expressed genes (DEGs) and molecular regulatory mechanisms related to adipogenic differentiation between IMF- and AbF-derived preadipocytes, we analysed the mRNA expression profiles in preadipocytes (0d, Pre-) and adipocytes (10d, Ad-) from IMF and AbF of Gushi chickens.
AbF-derived preadipocytes exhibited a higher adipogenic differentiation ability (96.4% + 0.6) than IMF-derived preadipocytes (86.0% + 0.4) (p < 0.01). By Ribo-Zero RNA sequencing, we obtained 4403 (2055 upregulated and 2348 downregulated) and 4693 (2797 upregulated and 1896 downregulated) DEGs between preadipocytes and adipocytes in the IMF and Ad groups, respectively. For IMF-derived preadipocyte differentiation, pathways related to the PPAR signalling pathway, ECM-receptor interaction and focal adhesion pathway were significantly enriched. For AbF-derived preadipocyte differentiation, the steroid biosynthesis pathways, calcium signaling pathway and ECM-receptor interaction pathway were significantly enriched. A large number of DEGs related to lipid metabolism, fatty acid metabolism and preadipocyte differentiation, such as PPARG, ACSBG2, FABP4, FASN, APOA1 and INSIG1, were identified in our study.
This study revealed large transcriptomic differences between IMF- and AbF-derived preadipocyte differentiation. A large number of DEGs and transcription factors that were closely related to fatty acid metabolism, lipid metabolism and preadipocyte differentiation were identified in the present study. Additionally, the microenvironment of IMF- and AbF-derived preadipocyte may play a significant role in adipogenic differentiation. This study provides valuable evidence to understand the molecular mechanisms underlying adipogenesis and fat deposition in chickens.
脂肪组织在身体不同部位的分布和沉积是影响鸡胴体质量和肉质的关键因素。肌肉内脂肪（IMF）含量是与肉质相关的重要因素，而腹部脂肪（AbF）被认为是影响家禽屠宰效率的主要因素之一。为了研究与 IMF 和 AbF 衍生前体脂肪细胞成脂分化相关的差异表达基因（DEGs）和分子调控机制，我们分析了来自固始鸡 IMF 和 AbF 的前体脂肪细胞（0d，Pre-）和脂肪细胞（10d，Ad-）的 mRNA 表达谱。
AbF 衍生的前体脂肪细胞的成脂分化能力（96.4%±0.6）高于 IMF 衍生的前体脂肪细胞（86.0%±0.4）（p<0.01）。通过 Ribo-Zero RNA 测序，我们分别在 IMF 和 Ad 组中获得了前体脂肪细胞和脂肪细胞之间的 4403 个（2055 个上调和 2348 个下调）和 4693 个（2797 个上调和 1896 个下调）差异表达基因。对于 IMF 衍生的前体脂肪细胞分化，与 PPAR 信号通路、ECM-受体相互作用和焦点黏附途径相关的途径显著富集。对于 AbF 衍生的前体脂肪细胞分化，类固醇生物合成途径、钙信号通路和 ECM-受体相互作用途径显著富集。在本研究中，我们鉴定了大量与脂质代谢、脂肪酸代谢和前体脂肪细胞分化相关的差异表达基因，如 PPARG、ACSBG2、FABP4、FASN、APOA1 和 INSIG1。
本研究揭示了 IMF 和 AbF 衍生前体脂肪细胞分化之间的大量转录组差异。在本研究中，鉴定了大量与脂肪酸代谢、脂质代谢和前体脂肪细胞分化密切相关的差异表达基因和转录因子。此外，IMF 和 AbF 衍生前体脂肪细胞的微环境可能在成脂分化中起重要作用。本研究为理解鸡脂肪生成和脂肪沉积的分子机制提供了有价值的证据。
